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• W2083375414 abstract "A new, modified culture method that utilizes a transwell plate with a 0.4 µm pore-size microporous membrane was developed. This system allows only trace nutrients from the soil into the liquid culture through the microporous membrane. The method is a more powerful tool for the discovery of novel species from soils than are traditional methods. Such newly identified species could potentially produce useful metabolites. A bacterial strain, T515 T , was isolated using this modified culture method. Growth of strain T515 T occurred at pH 4–9 in a temperature range between 20 °C and 40 °C and in the presence of 0–2 % (w/v) NaCl on R2A agar. Colonies on the agar plates were tiny, white, and convex after 5 days incubation at 28 °C. Comparative analysis of the nearly full-length 16S rRNA gene sequence of strain T515 T revealed close pairwise similarity with species of the genus Bacillus , and strain T515 T was most closely related to Bacillus panaciterrae Gsoil 1517 T (96.7 %) and Bacillus funiculus NAF001 T (96.0 %). The major quinone of strain T515 T was menaquinone-7 (MK-7) and the major fatty acids were iso-C 15 : 0 (45.5 %), anteiso-C 15 : 0 (23.2 %) and C 16 : 0 (10.9 %). The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Strain T515 T was sensitive to streptomycin and tetracycline, but resistant to rifampicin (0.125 µg ml −1 ), ampicillin (0.5 µg ml −1 ) and chloramphenicol (1 µg ml −1 ). The strain showed antimicrobial activities against the six strains tested: Bacillus subtilis KEMB 51201-001, Staphylococcus aureus KEMB 4659, Pseudomonas aeruginosa KACC 10185, Staphylococcus epidermidis KACC 13234, Paenibacillus larvae KACC 14031 and Escherichia coli KEMB 212-234. Based on these results, strain T515 T represents a novel species of the genus Bacillus with the proposed name, Bacillus polymachus sp. nov. The type strain is T515 T ( = KEMB 9005-168 T = KACC 18242 T = NBRC 110614 T )." @default.
• W2083375414 created "2016-06-24" @default.
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• W2083375414 date "2015-02-01" @default.
• W2083375414 modified "2023-09-26" @default.
• W2083375414 title "Bacillus polymachus sp. nov., with a broad range of antibacterial activity, isolated from forest topsoil samples by using a modified culture method" @default.
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• W2083375414 doi "https://doi.org/10.1099/ijs.0.070326-0" @default.
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