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- W2083377413 abstract "Direct UV detection of carbohydrates in free solution capillary electrophoresis at 270 nm is made possible by a photo-oxidation reaction. Glucose, rhamnose and xylose were shown to have unique UV absorption spectra hypothesizing different UV absorbing intermediates for their respective photo-oxidation. NMR spectroscopy of the photo-oxidation end products proved they consisted of carboxylates and not malondialdehyde as previously theorized and that oxygen thus plays a key role in the photo-oxidation pathway. Adding the photo-initiator Irgacure® 2959 in the background electrolyte increased sensitivity by 40% at an optimum concentration of 1 × 10−4 mM and 1 × 10−8 mM for conventional 50 μm i.d. capillaries and for the corresponding extended light path capillaries, respectively." @default.
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- W2083377413 date "2014-01-01" @default.
- W2083377413 modified "2023-10-18" @default.
- W2083377413 title "Understanding and improving direct UV detection of monosaccharides and disaccharides in free solution capillary electrophoresis" @default.
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- W2083377413 doi "https://doi.org/10.1016/j.aca.2013.12.001" @default.
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