FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2083419051> ?p ?o ?g. }

• W2083419051 endingPage "12127" @default.
• W2083419051 startingPage "12119" @default.
• W2083419051 abstract "A recently reported variant of human transthyretin (TTR), Leu-55-Pro, implicated as the causative agent in early-onset familial amyloid polyneuropathy was expressed and characterized, and its denaturation pathway and amyloidogenicity were compared to those of wild-type transthyretin. The overlap-extension polymerase chain reaction (PCR) methodology was used to introduce the Leu-55-Pro mutation into the transthyretin DNA sequence and to construct a new expression system. The Leu-55-Pro variant of transthyretin was expressed with a leader sequence to ensure secretion into the periplasmic space of Escherichia coli. Transthyretin's resistance to sodium dodecyl sulfate- (SDS-) induced denaturation was utilized to measure the quaternary stability as a function of pH employing SDS-polyacrylamide gel electrophoresis (PAGE) in the presence and absence of an amyloid fibril inhibitor, Z 3-14. These studies reveal that the Leu-55-Pro TTR tetramer is significantly less stable than wild-type TTR. This is relevant because we have previously shown that the partial denaturation of transthyretin is sufficient to effect amyloid fibril formation from a denaturation intermediate which may be a structured monomer. The ability of Leu-55-Pro TTR to denature to the amyloidogenic intermediate at pHs where the wild-type protein is stable may explain the variant's propensity to form amyloid fibrils in vitro and in vivo where the wild-type protein remains stable and nonamyloidogenic. Congo red binding, polarized light microscopy, and electron microscopy confirm the characteristic structure of amyloid fibrils produced from Leu-55-Pro TTR in vitro.(ABSTRACT TRUNCATED AT 250 WORDS)" @default.
• W2083419051 created "2016-06-24" @default.
• W2083419051 creator A5005154580 @default.
• W2083419051 creator A5036860380 @default.
• W2083419051 creator A5081499831 @default.
• W2083419051 date "1993-11-01" @default.
• W2083419051 modified "2023-10-16" @default.
• W2083419051 title "Transthyretin mutation Leu-55-Pro significantly alters tetramer stability and increases amyloidogenicity" @default.
• W2083419051 cites W1243723833 @default.
• W2083419051 cites W1517283541 @default.
• W2083419051 cites W1517296610 @default.
• W2083419051 cites W1533421793 @default.
• W2083419051 cites W1535133733 @default.
• W2083419051 cites W1551211932 @default.
• W2083419051 cites W1560285331 @default.
• W2083419051 cites W1568589356 @default.
• W2083419051 cites W1570203195 @default.
• W2083419051 cites W1582439177 @default.
• W2083419051 cites W1749557808 @default.
• W2083419051 cites W1967144890 @default.
• W2083419051 cites W1973241521 @default.
• W2083419051 cites W1980469335 @default.
• W2083419051 cites W1986792544 @default.
• W2083419051 cites W1988478867 @default.
• W2083419051 cites W2011606718 @default.
• W2083419051 cites W2016778798 @default.
• W2083419051 cites W2022945618 @default.
• W2083419051 cites W2027453903 @default.
• W2083419051 cites W2028501050 @default.
• W2083419051 cites W2028545388 @default.
• W2083419051 cites W2042159493 @default.
• W2083419051 cites W2046164755 @default.
• W2083419051 cites W2058110454 @default.
• W2083419051 cites W2060913542 @default.
• W2083419051 cites W2064146660 @default.
• W2083419051 cites W2066574980 @default.
• W2083419051 cites W2076467891 @default.
• W2083419051 cites W2100837269 @default.
• W2083419051 cites W2101108802 @default.
• W2083419051 cites W2133783264 @default.
• W2083419051 cites W2137399373 @default.
• W2083419051 cites W2152278845 @default.
• W2083419051 cites W2161273461 @default.
• W2083419051 cites W2197268372 @default.
• W2083419051 cites W2197678796 @default.
• W2083419051 cites W2341371491 @default.
• W2083419051 cites W2410896815 @default.
• W2083419051 cites W2414304308 @default.
• W2083419051 cites W973302723 @default.
• W2083419051 doi "https://doi.org/10.1021/bi00096a024" @default.
• W2083419051 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8218290" @default.
• W2083419051 hasPublicationYear "1993" @default.
• W2083419051 type Work @default.
• W2083419051 sameAs 2083419051 @default.
• W2083419051 citedByCount "185" @default.
• W2083419051 countsByYear W20834190512012 @default.
• W2083419051 countsByYear W20834190512013 @default.
• W2083419051 countsByYear W20834190512014 @default.
• W2083419051 countsByYear W20834190512015 @default.
• W2083419051 countsByYear W20834190512016 @default.
• W2083419051 countsByYear W20834190512017 @default.
• W2083419051 countsByYear W20834190512018 @default.
• W2083419051 countsByYear W20834190512019 @default.
• W2083419051 countsByYear W20834190512020 @default.
• W2083419051 countsByYear W20834190512021 @default.
• W2083419051 countsByYear W20834190512022 @default.
• W2083419051 countsByYear W20834190512023 @default.
• W2083419051 crossrefType "journal-article" @default.
• W2083419051 hasAuthorship W2083419051A5005154580 @default.
• W2083419051 hasAuthorship W2083419051A5036860380 @default.
• W2083419051 hasAuthorship W2083419051A5081499831 @default.
• W2083419051 hasConcept C104317684 @default.
• W2083419051 hasConcept C116084860 @default.
• W2083419051 hasConcept C121157162 @default.
• W2083419051 hasConcept C134018914 @default.
• W2083419051 hasConcept C13965031 @default.
• W2083419051 hasConcept C142724271 @default.
• W2083419051 hasConcept C143065580 @default.
• W2083419051 hasConcept C153911025 @default.
• W2083419051 hasConcept C176944494 @default.
• W2083419051 hasConcept C179104552 @default.
• W2083419051 hasConcept C181199279 @default.
• W2083419051 hasConcept C185592680 @default.
• W2083419051 hasConcept C27523624 @default.
• W2083419051 hasConcept C2776923230 @default.
• W2083419051 hasConcept C2777633098 @default.
• W2083419051 hasConcept C2778886173 @default.
• W2083419051 hasConcept C2778896982 @default.
• W2083419051 hasConcept C2779134260 @default.
• W2083419051 hasConcept C2779951007 @default.
• W2083419051 hasConcept C502032728 @default.
• W2083419051 hasConcept C55493867 @default.
• W2083419051 hasConcept C71924100 @default.
• W2083419051 hasConcept C86803240 @default.
• W2083419051 hasConceptScore W2083419051C104317684 @default.
• W2083419051 hasConceptScore W2083419051C116084860 @default.
• W2083419051 hasConceptScore W2083419051C121157162 @default.
• W2083419051 hasConceptScore W2083419051C134018914 @default.

• next