Matches in SemOpenAlex for { <https://semopenalex.org/work/W2083439095> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W2083439095 endingPage "405" @default.
- W2083439095 startingPage "397" @default.
- W2083439095 abstract "Objective: To investigate the feasibility of temporally and spatially restricted Kringle5 expression induced by radiation, as well as the dual effect of radiotherapy and antiangiogenic therapy in lung adenocarcinoma in vitro. Methods: We first constructed recombinant baculovirus vectors containing Egr1 promoter and human plasminogen Kringle5 gene (rhK5), then transfected them into lung adenocarcinoma cells (A549). Transfect efficiency of the baculovirus for gene transfer in A549 cells and the activity of Egr1 promoter induced by X-radiation were detected by fluorescence microscopy. The rhK5 mRNA transcription and rhK5 protein expression were detected by Real-time PCR and Western blot assay, respectively. The apoptosis asssay of human umbilical veins endothelial cells (HUVEC) was analyzed by flow cytometry. Results: The recombinant baculovirus were successfully transfected into A549 and HUVEC cells. As for the temporal regulation, the rhK5 mRNA transcription and rhK5 protein expression were elevated with the irradiation time significantly. And the HUVEC apoptotic percentage increased in relation to the irradiation time as well. As for the spatial regulation, rhK5 mRNA transcription level of A549 cell lines transfected with recombinant baculovirus Egr1-K5 was significantly higher than that of control groups after the same dose of X-radiation. When we analyzed the dose and frequency of X-radiation, no difference was observed among each dose after continuously three-times of irradiation. Conclusion: Baculovirus-mediated Egr1-K5 can be used in gene radiotherapy for its temporary and spatial controllable rhK5 expression by X-radiation and the consequent HUVEC apoptosis in vitro study. And low dose and more times of irradiation might be more effective. It would provide a promising way for the tumor treatment." @default.
- W2083439095 created "2016-06-24" @default.
- W2083439095 creator A5015365923 @default.
- W2083439095 creator A5018740595 @default.
- W2083439095 creator A5030785854 @default.
- W2083439095 date "2012-01-01" @default.
- W2083439095 modified "2023-10-05" @default.
- W2083439095 title "Baculovirus Mediated Experimental Research on Targeted Egr1-Kringle 5 Gene Radiotherapy in Lung Adenocarcinoma" @default.
- W2083439095 cites W107028948 @default.
- W2083439095 cites W1586102679 @default.
- W2083439095 cites W1965478720 @default.
- W2083439095 cites W1975519085 @default.
- W2083439095 cites W1976878784 @default.
- W2083439095 cites W1980911614 @default.
- W2083439095 cites W2003026664 @default.
- W2083439095 cites W2024758368 @default.
- W2083439095 cites W2029839079 @default.
- W2083439095 cites W2048454485 @default.
- W2083439095 cites W2051974869 @default.
- W2083439095 cites W2057537794 @default.
- W2083439095 cites W2063132642 @default.
- W2083439095 cites W2066644385 @default.
- W2083439095 cites W2076003033 @default.
- W2083439095 cites W2081586476 @default.
- W2083439095 cites W2085482078 @default.
- W2083439095 cites W2155922793 @default.
- W2083439095 cites W2158898242 @default.
- W2083439095 cites W2161707475 @default.
- W2083439095 cites W2165277386 @default.
- W2083439095 cites W240104262 @default.
- W2083439095 cites W60698725 @default.
- W2083439095 doi "https://doi.org/10.4236/jct.2012.324052" @default.
- W2083439095 hasPublicationYear "2012" @default.
- W2083439095 type Work @default.
- W2083439095 sameAs 2083439095 @default.
- W2083439095 citedByCount "0" @default.
- W2083439095 crossrefType "journal-article" @default.
- W2083439095 hasAuthorship W2083439095A5015365923 @default.
- W2083439095 hasAuthorship W2083439095A5018740595 @default.
- W2083439095 hasAuthorship W2083439095A5030785854 @default.
- W2083439095 hasBestOaLocation W20834390951 @default.
- W2083439095 hasConcept C104317684 @default.
- W2083439095 hasConcept C111599444 @default.
- W2083439095 hasConcept C150194340 @default.
- W2083439095 hasConcept C153911025 @default.
- W2083439095 hasConcept C190283241 @default.
- W2083439095 hasConcept C2776415932 @default.
- W2083439095 hasConcept C2780600689 @default.
- W2083439095 hasConcept C502942594 @default.
- W2083439095 hasConcept C54009773 @default.
- W2083439095 hasConcept C54355233 @default.
- W2083439095 hasConcept C553184892 @default.
- W2083439095 hasConcept C55493867 @default.
- W2083439095 hasConcept C81729549 @default.
- W2083439095 hasConcept C81885089 @default.
- W2083439095 hasConcept C86803240 @default.
- W2083439095 hasConceptScore W2083439095C104317684 @default.
- W2083439095 hasConceptScore W2083439095C111599444 @default.
- W2083439095 hasConceptScore W2083439095C150194340 @default.
- W2083439095 hasConceptScore W2083439095C153911025 @default.
- W2083439095 hasConceptScore W2083439095C190283241 @default.
- W2083439095 hasConceptScore W2083439095C2776415932 @default.
- W2083439095 hasConceptScore W2083439095C2780600689 @default.
- W2083439095 hasConceptScore W2083439095C502942594 @default.
- W2083439095 hasConceptScore W2083439095C54009773 @default.
- W2083439095 hasConceptScore W2083439095C54355233 @default.
- W2083439095 hasConceptScore W2083439095C553184892 @default.
- W2083439095 hasConceptScore W2083439095C55493867 @default.
- W2083439095 hasConceptScore W2083439095C81729549 @default.
- W2083439095 hasConceptScore W2083439095C81885089 @default.
- W2083439095 hasConceptScore W2083439095C86803240 @default.
- W2083439095 hasIssue "04" @default.
- W2083439095 hasLocation W20834390951 @default.
- W2083439095 hasOpenAccess W2083439095 @default.
- W2083439095 hasPrimaryLocation W20834390951 @default.
- W2083439095 hasRelatedWork W2351429383 @default.
- W2083439095 hasRelatedWork W2355678225 @default.
- W2083439095 hasRelatedWork W2358299559 @default.
- W2083439095 hasRelatedWork W2359858375 @default.
- W2083439095 hasRelatedWork W2375446097 @default.
- W2083439095 hasRelatedWork W2376866590 @default.
- W2083439095 hasRelatedWork W2384824869 @default.
- W2083439095 hasRelatedWork W2410949163 @default.
- W2083439095 hasRelatedWork W2555524919 @default.
- W2083439095 hasRelatedWork W4283592292 @default.
- W2083439095 hasVolume "03" @default.
- W2083439095 isParatext "false" @default.
- W2083439095 isRetracted "false" @default.
- W2083439095 magId "2083439095" @default.
- W2083439095 workType "article" @default.