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• W2083445384 endingPage "279" @default.
• W2083445384 startingPage "267" @default.
• W2083445384 abstract "Several species of DNA molecules are packaged into λ phage heads if they carry the region around the cohesive end site of λ phage ( cos λ). The minimal functional sequence around cos λ needed for packaging was examined by cloning in pBR322. The results showed that the minimal region contained 85 bp around cos λ 45 bp of the left arm of λ phage and 40 bp of the right arm. A 75-bp region located to the right of the minimal region seems to enhance packaging. A 223-bp fragment containing these regions can be used as a portable element for plasmid DNA packaging into λ phage heads. Plasmid ppBest 322, a derivative of pBR322 carrying this portable packager and both amp and tet genes, was constructed. This plasmid is useful for cloning of large DNA fragments." @default.
• W2083445384 created "2016-06-24" @default.
• W2083445384 creator A5045956357 @default.
• W2083445384 creator A5050702441 @default.
• W2083445384 date "1982-12-01" @default.
• W2083445384 modified "2023-10-04" @default.
• W2083445384 title "Identification of sequences necessary for packaging DNA into lambda phage heads" @default.
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• W2083445384 doi "https://doi.org/10.1016/0378-1119(82)90045-2" @default.
• W2083445384 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6299893" @default.
• W2083445384 hasPublicationYear "1982" @default.
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