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- W2083460901 abstract "Identification of chloroplast coupling factor particles, by the freeze-etching and negative-staining techniques, was made utilizing chloroplast thylakoids isolated from spinach leaves. Complete removal of particles, comparable in diameter to purified coupling factor particles, from the outer surface of freeze-etched thylakoids was achieved by treatment with 0.8% silicotungstate. Reappearance of particles, comparable in diameter to purified coupling factor particles, on the outer surface of freeze-etched thylakoids was demonstrated by combining silicotungstate-treated thylakoids with purified chloroplast coupling factor. Negative-staining results were in agreement with the freeze-etch data. The results demonstrate that the chloroplast coupling factor particles are exposed on the outer surface." @default.
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- W2083460901 date "1974-10-01" @default.
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- W2083460901 title "IDENTIFICATION OF CHLOROPLAST COUPLING FACTOR BY FREEZE-ETCHING AND NEGATIVE-STAINING TECHNIQUES" @default.
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- W2083460901 doi "https://doi.org/10.1083/jcb.63.1.24" @default.
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