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• W2083490429 abstract "In the present study, ultrasensitive detection of Ag(+) is demonstrated by a biocatalytic signal amplification system which is realized by only one DNA sequence based electrical contacted enzyme structure and the Au nanoparticles/Carbon nanodots (AuNPs/C-dots) composite immobilization platform. In the presence of Ag(+), with the interaction of cytosine-Ag(+)-cytosine (C-Ag(+)-C), cytosine-rich DNA sequence labeled with methylene blue (MB) molecules near 5' end and Glucose Oxidase (GOx) at 3' end, has a self-hybridization and then forms a duplex-like structure which makes MB and GOx approach the AuNPs/C-dots modified electrode. MB units can then act as a relay that electrically contacts GOx with the AuNPs/C-dots modified electrode and activate the bioelectrocatalyzed oxidation of glucose to glucose acid. In consequence, based on the bioelectrocatalyzed signal amplification on the AuNPs/C-dots platform, Ag(+) could be quantitatively detected in the range of 10(-11)-10(-5) M with a low detection limit of 3 pM. Also, there is an excellent selectivity against other interferential metal ions. The detection of Ag(+) ions was realized by Ag(+) self-induced conformational change of DNA scaffold which involved only one oligonucleotide showing its convenience and availability." @default.
• W2083490429 created "2016-06-24" @default.
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• W2083490429 date "2014-09-01" @default.
• W2083490429 modified "2023-09-27" @default.
• W2083490429 title "Amplified and selective detection of Ag+ ions based on electrically contacted enzymes on duplex-like DNA scaffolds" @default.
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• W2083490429 doi "https://doi.org/10.1016/j.bios.2014.03.017" @default.
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