FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2083493148> ?p ?o ?g. }

• W2083493148 endingPage "855" @default.
• W2083493148 startingPage "849" @default.
• W2083493148 abstract "T cell receptor (TCR) gene adoptive therapy is a promising clinical approach for the treatment of malignant tumors and viral diseases. However, the effectiveness of this strategy is hampered by the generation of mixed TCR heterodimers comprising both exogenous and endogenous TCR chains (i.e., mispairing of TCR chains). In the present study, we constructed genetically encoded reporters fused to a pair of fluorescent proteins [enhanced cyan fluorescent protein (ECFP)/enhanced yellow fluorescent protein (EYFP)] to monitor the expression of TCRαζβζ and pairing between TCRαζ and TCRβζ. We demonstrate that these reporters provide accurate images of TCRαζβζ expression, which is markedly stronger with evident microclusters accumulated at the plasma membrane compared to wild-type (wt)TCR. Using fluorescence resonance energy transfer (FRET) analysis, we demonstrate that, in addition to the improved pairing, the expression and assembly of TCRαζβζ are independent of endogenous CD3 subunits. These results suggest that the fusion genes, TCRαζ and TCRβζ, coupled to ECFP and EYFP, respectively can effectively monitor the expression and interaction in cells. Our data suggest a novel strategy with which it is possible to effectively express and pair TCRαζβζ, thus making TCR gene adoptive therapy more effective." @default.
• W2083493148 created "2016-06-24" @default.
• W2083493148 creator A5010599695 @default.
• W2083493148 creator A5023308455 @default.
• W2083493148 creator A5027467135 @default.
• W2083493148 creator A5033859479 @default.
• W2083493148 creator A5069811393 @default.
• W2083493148 creator A5070962397 @default.
• W2083493148 creator A5074385560 @default.
• W2083493148 creator A5079804745 @default.
• W2083493148 date "2014-07-08" @default.
• W2083493148 modified "2023-10-18" @default.
• W2083493148 title "Imaging of T-cell receptor fused to CD3ζ reveals enhanced expression and improved pairing in living cells" @default.
• W2083493148 cites W1512812674 @default.
• W2083493148 cites W1530695298 @default.
• W2083493148 cites W1655693035 @default.
• W2083493148 cites W1663561827 @default.
• W2083493148 cites W1966496772 @default.
• W2083493148 cites W1976101370 @default.
• W2083493148 cites W1978670809 @default.
• W2083493148 cites W1984446137 @default.
• W2083493148 cites W1994872258 @default.
• W2083493148 cites W2000303800 @default.
• W2083493148 cites W2001624434 @default.
• W2083493148 cites W2007605965 @default.
• W2083493148 cites W2029909479 @default.
• W2083493148 cites W2050302632 @default.
• W2083493148 cites W2077200917 @default.
• W2083493148 cites W2090648593 @default.
• W2083493148 cites W2092114945 @default.
• W2083493148 cites W2094652659 @default.
• W2083493148 cites W2098249455 @default.
• W2083493148 cites W2098432967 @default.
• W2083493148 cites W2103930728 @default.
• W2083493148 cites W2106083290 @default.
• W2083493148 cites W2121043133 @default.
• W2083493148 cites W2131553403 @default.
• W2083493148 cites W2154008781 @default.
• W2083493148 cites W2161402514 @default.
• W2083493148 doi "https://doi.org/10.3892/ijmm.2014.1839" @default.
• W2083493148 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25017903" @default.
• W2083493148 hasPublicationYear "2014" @default.
• W2083493148 type Work @default.
• W2083493148 sameAs 2083493148 @default.
• W2083493148 citedByCount "4" @default.
• W2083493148 countsByYear W20834931482016 @default.
• W2083493148 countsByYear W20834931482017 @default.
• W2083493148 countsByYear W20834931482019 @default.
• W2083493148 countsByYear W20834931482023 @default.
• W2083493148 crossrefType "journal-article" @default.
• W2083493148 hasAuthorship W2083493148A5010599695 @default.
• W2083493148 hasAuthorship W2083493148A5023308455 @default.
• W2083493148 hasAuthorship W2083493148A5027467135 @default.
• W2083493148 hasAuthorship W2083493148A5033859479 @default.
• W2083493148 hasAuthorship W2083493148A5069811393 @default.
• W2083493148 hasAuthorship W2083493148A5070962397 @default.
• W2083493148 hasAuthorship W2083493148A5074385560 @default.
• W2083493148 hasAuthorship W2083493148A5079804745 @default.
• W2083493148 hasBestOaLocation W20834931481 @default.
• W2083493148 hasConcept C104317684 @default.
• W2083493148 hasConcept C114684123 @default.
• W2083493148 hasConcept C121332964 @default.
• W2083493148 hasConcept C142613039 @default.
• W2083493148 hasConcept C147483822 @default.
• W2083493148 hasConcept C153911025 @default.
• W2083493148 hasConcept C167672396 @default.
• W2083493148 hasConcept C19317047 @default.
• W2083493148 hasConcept C2776090121 @default.
• W2083493148 hasConcept C2778067846 @default.
• W2083493148 hasConcept C54355233 @default.
• W2083493148 hasConcept C62520636 @default.
• W2083493148 hasConcept C86803240 @default.
• W2083493148 hasConcept C8891405 @default.
• W2083493148 hasConcept C91881484 @default.
• W2083493148 hasConcept C95444343 @default.
• W2083493148 hasConcept C96305047 @default.
• W2083493148 hasConceptScore W2083493148C104317684 @default.
• W2083493148 hasConceptScore W2083493148C114684123 @default.
• W2083493148 hasConceptScore W2083493148C121332964 @default.
• W2083493148 hasConceptScore W2083493148C142613039 @default.
• W2083493148 hasConceptScore W2083493148C147483822 @default.
• W2083493148 hasConceptScore W2083493148C153911025 @default.
• W2083493148 hasConceptScore W2083493148C167672396 @default.
• W2083493148 hasConceptScore W2083493148C19317047 @default.
• W2083493148 hasConceptScore W2083493148C2776090121 @default.
• W2083493148 hasConceptScore W2083493148C2778067846 @default.
• W2083493148 hasConceptScore W2083493148C54355233 @default.
• W2083493148 hasConceptScore W2083493148C62520636 @default.
• W2083493148 hasConceptScore W2083493148C86803240 @default.
• W2083493148 hasConceptScore W2083493148C8891405 @default.
• W2083493148 hasConceptScore W2083493148C91881484 @default.
• W2083493148 hasConceptScore W2083493148C95444343 @default.
• W2083493148 hasConceptScore W2083493148C96305047 @default.
• W2083493148 hasIssue "3" @default.
• W2083493148 hasLocation W20834931481 @default.
• W2083493148 hasLocation W20834931482 @default.
• W2083493148 hasOpenAccess W2083493148 @default.
• W2083493148 hasPrimaryLocation W20834931481 @default.

• next