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- W2083528282 abstract "Long-term stability is an essential requirement for all international biological standards. The main stocks of the current international standards for thromboplastin, i.e. RBT/05 (rabbit brain thromboplastin) and rTF/09 (recombinant human tissue factor), are stored at -20°C. The aim of the present study is to assess the long-term stability of the international sensitivity index (ISI) for RBT/05 and rTF/09.Part of the main stocks of RBT/05 and rTF/09 were stored at -70°C and -150°C, up to 38months. At various time points samples were taken from the materials stored at -20°C, -70°C, and -150°C. The samples were reconstituted and analysed in the prothrombin time (PT) test using plasma samples derived from healthy subjects and patients treated with vitamin K-antagonists (VKA). The PT's obtained with the standards stored at -20°C were compared to the PT's obtained with the standards stored at -70°C and at -150°C. The PT's were used to calculate relative ISI values by means of orthogonal regression.There were no important differences between the ISI values for the materials stored at -20°C, -70°C, and -150°C. There was no significant trend with storage time.The ISI values for the international standards RBT/05 and rTF/09 appear to be stable at storage temperatures of -20°C, -70°C, and -150°C." @default.
- W2083528282 created "2016-06-24" @default.
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- W2083528282 date "2015-07-01" @default.
- W2083528282 modified "2023-09-26" @default.
- W2083528282 title "Long-term stability of international standards for thromboplastin stored at -20°C, -70°C, and -150°C" @default.
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- W2083528282 doi "https://doi.org/10.1016/j.thromres.2015.04.033" @default.
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