Matches in SemOpenAlex for { <https://semopenalex.org/work/W2083550005> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W2083550005 endingPage "89" @default.
- W2083550005 startingPage "83" @default.
- W2083550005 abstract "Polyethylene glycol (PEG) is extensively employed for protein purification by fractional precipitation. Efficiency of precipitation is highest when the solution pH is near the isoelectric point of the target protein. At pH values far from the isoelectric point of the target protein, proteins develop a net positive or negative charge and are more resistant to precipitation. We have found that divalent cations (Ba2+, Sr2+, and Ca2+) or divalent anions (SO42−) significantly change the pattern of PEG precipitation when the ion is chosen so as to counteract the expected net charge on the target protein. At moderate (5–50 mm) concentrations of Ba2+, negatively charged proteins can be precipitated from solution at pH values as high as 10 with efficiency unchanged from precipitation at pH values near their isoelectric point values. The mechanism of PEG precipitation of protein at these high pH values appears to be unchanged from the mechanism operative at the protein isoelectric point. Precipitation is rapid and the capacity for protein precipitation is high. There is no detectable coprecipitation of small molecules (AMP, ATP, and NADH) or soluble proteins (carbonic anhydrase) induced when large quantities of protein are precipitated by this method. The purification of bovine carbonic anhydrase from erythrocyte lysate is more efficient at pH 10 in the presence of Ba2+ than is conventional PEG precipitation carried out at the isoelectric point of carbonic anhydrase. Application of these observations should broaden the utility of protein purification by fractional precipitation with PEG." @default.
- W2083550005 created "2016-06-24" @default.
- W2083550005 creator A5037689932 @default.
- W2083550005 creator A5061650249 @default.
- W2083550005 creator A5083015210 @default.
- W2083550005 date "1991-02-01" @default.
- W2083550005 modified "2023-09-27" @default.
- W2083550005 title "Effect of divalent ions on protein precipitation with polyethylene glycol: Mechanism of action and applications" @default.
- W2083550005 cites W1487060942 @default.
- W2083550005 cites W2026882610 @default.
- W2083550005 cites W2085656571 @default.
- W2083550005 doi "https://doi.org/10.1016/1046-5928(91)90015-b" @default.
- W2083550005 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1821777" @default.
- W2083550005 hasPublicationYear "1991" @default.
- W2083550005 type Work @default.
- W2083550005 sameAs 2083550005 @default.
- W2083550005 citedByCount "10" @default.
- W2083550005 countsByYear W20835500052013 @default.
- W2083550005 countsByYear W20835500052015 @default.
- W2083550005 countsByYear W20835500052020 @default.
- W2083550005 countsByYear W20835500052021 @default.
- W2083550005 countsByYear W20835500052022 @default.
- W2083550005 crossrefType "journal-article" @default.
- W2083550005 hasAuthorship W2083550005A5037689932 @default.
- W2083550005 hasAuthorship W2083550005A5061650249 @default.
- W2083550005 hasAuthorship W2083550005A5083015210 @default.
- W2083550005 hasConcept C10138342 @default.
- W2083550005 hasConcept C10390740 @default.
- W2083550005 hasConcept C107054158 @default.
- W2083550005 hasConcept C121332964 @default.
- W2083550005 hasConcept C153294291 @default.
- W2083550005 hasConcept C162324750 @default.
- W2083550005 hasConcept C178790620 @default.
- W2083550005 hasConcept C181199279 @default.
- W2083550005 hasConcept C185592680 @default.
- W2083550005 hasConcept C2776964284 @default.
- W2083550005 hasConcept C43617362 @default.
- W2083550005 hasConcept C54400483 @default.
- W2083550005 hasConcept C55493867 @default.
- W2083550005 hasConcept C57409179 @default.
- W2083550005 hasConcept C74774607 @default.
- W2083550005 hasConcept C74884574 @default.
- W2083550005 hasConcept C8985478 @default.
- W2083550005 hasConcept C96294017 @default.
- W2083550005 hasConceptScore W2083550005C10138342 @default.
- W2083550005 hasConceptScore W2083550005C10390740 @default.
- W2083550005 hasConceptScore W2083550005C107054158 @default.
- W2083550005 hasConceptScore W2083550005C121332964 @default.
- W2083550005 hasConceptScore W2083550005C153294291 @default.
- W2083550005 hasConceptScore W2083550005C162324750 @default.
- W2083550005 hasConceptScore W2083550005C178790620 @default.
- W2083550005 hasConceptScore W2083550005C181199279 @default.
- W2083550005 hasConceptScore W2083550005C185592680 @default.
- W2083550005 hasConceptScore W2083550005C2776964284 @default.
- W2083550005 hasConceptScore W2083550005C43617362 @default.
- W2083550005 hasConceptScore W2083550005C54400483 @default.
- W2083550005 hasConceptScore W2083550005C55493867 @default.
- W2083550005 hasConceptScore W2083550005C57409179 @default.
- W2083550005 hasConceptScore W2083550005C74774607 @default.
- W2083550005 hasConceptScore W2083550005C74884574 @default.
- W2083550005 hasConceptScore W2083550005C8985478 @default.
- W2083550005 hasConceptScore W2083550005C96294017 @default.
- W2083550005 hasIssue "1" @default.
- W2083550005 hasLocation W20835500051 @default.
- W2083550005 hasLocation W20835500052 @default.
- W2083550005 hasOpenAccess W2083550005 @default.
- W2083550005 hasPrimaryLocation W20835500051 @default.
- W2083550005 hasRelatedWork W2013526331 @default.
- W2083550005 hasRelatedWork W2017687723 @default.
- W2083550005 hasRelatedWork W2037651561 @default.
- W2083550005 hasRelatedWork W2045939673 @default.
- W2083550005 hasRelatedWork W2083550005 @default.
- W2083550005 hasRelatedWork W2150895058 @default.
- W2083550005 hasRelatedWork W2346614825 @default.
- W2083550005 hasRelatedWork W2386994050 @default.
- W2083550005 hasRelatedWork W2467415319 @default.
- W2083550005 hasRelatedWork W2737787551 @default.
- W2083550005 hasVolume "2" @default.
- W2083550005 isParatext "false" @default.
- W2083550005 isRetracted "false" @default.
- W2083550005 magId "2083550005" @default.
- W2083550005 workType "article" @default.