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- W2083582912 abstract "This paper aimed to develop a simple, sensitive, and rapid chromatographic procedure for the simultaneous analysis of human insulin and its main decomposition product using isocratic RP-HPLC/UV. A column type RP-C18 (100 × 4.6 mm, 3 μ m particle size, and pore size 130 Å) was used. o -Nitrophenol was used as internal standard. The eluent consists of 62% KH 2 PO 4 buffer (0.1 M), 26% ACN, and 12% MeOH. The final pH was adjusted to 3.1. The eluent was pumped at a flow rate of 1.0 mL/min and the effluent was monitored using DAD detector at 214 nm. The method produces a linear response over the concentration range of 0.0106 to 0.6810 mg/mL with detection limit of 0.0029 mg/mL. Considering the specifications of this method, the system was found to be suitable for rapid, direct routine analysis and stability studies of insulin." @default.
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- W2083582912 date "2014-07-23" @default.
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- W2083582912 title "A Rapid, Isocratic HPLC Method for Determination of Insulin and Its Degradation Product" @default.
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- W2083582912 doi "https://doi.org/10.1155/2014/749823" @default.
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