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• W2083595532 abstract "Anti-cytomegalovirus (CMV) antibodies reduce the incidence of CMV transmission and ameliorate the severity of CMV-associated disease. Neutralizing activity, measured as the ability of antibodies to prevent entry of cell-free virus, is an important component of natural immunity. However, in vivo CMV amplification may occur mainly via spread between adjacent cells within tissues. Thus, inhibition of cell-to-cell spread may be important when evaluating therapeutic antibodies or humoral responses to infection or immunization. In vitro CMV cell-to-cell spread is largely resistant to antibodies in fibroblast cultures but sensitive in endothelial cell cultures. In the present study antibodies in CMV hyperimmuneglobulin or seropositive human sera inhibited CMV cell-to-cell spread in epithelial cell cultures. Spread inhibition activity was quantitated with a GFP reporter assay employing GFP-tagged epithelialtropic variants of CMV strains Towne or AD169. Measurement of spread inhibition provides an additional parameter for the evaluation of candidate vaccines or immunotherapeutics and to further characterize the role of antibodies in controlling CMV transmission and disease." @default.
• W2083595532 created "2016-06-24" @default.
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• W2083595532 date "2013-09-01" @default.
• W2083595532 modified "2023-10-17" @default.
• W2083595532 title "Antibody inhibition of human cytomegalovirus spread in epithelial cell cultures" @default.
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• W2083595532 doi "https://doi.org/10.1016/j.jviromet.2013.04.015" @default.
• W2083595532 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3774129" @default.
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