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- W2083647986 abstract "Summary Under the conditions of our experiments we have found that dimethyl sulfoxide (DMSO) is toxic to white blood cells. Regardless of the cell type, the toxicity is more pronounced when the cells are exposed at room temperature (20 to 22°C) than at 4°C. The toxicity progressively increases with the time of exposure to DMSO and, among the cells studied, the toxicity of DMSO is more pronounced for mature granulocytes and monocytes. We have found that, regardless of cell type, 12.5% DMSO is the most desirable concentration for preservation of all white blood cells. We have also found that leukocytes do not preserve equally well. Among the acute leukemias, the more primitive the preserved cell is, the better is the degree of preservation. The reticulum cells, the most primitive leukemic cells tested, gave us the highest degree of preservation, followed by myeloblasts, monoblasts, and lymphosarcoma cells. Among the mature cells, lymphocytes both from normal individuals and from patients with chronic lymphatic leukemic can be successfully preserved, in direct contrast with mature granulocytes and monocytes, which are poorly preserved. Glucose added to the preserving media, at concentrations of 0.5 to 1.0 g%, increases the degree of preservation. Centrifugation seems to have a deleterious effect on the recovery of viable cells. Under the conditions of our experiment, cells were equally well preserved up to periods of 6 months." @default.
- W2083647986 created "2016-06-24" @default.
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- W2083647986 date "1967-05-01" @default.
- W2083647986 modified "2023-09-25" @default.
- W2083647986 title "Preservation of human normal and leukemic cells with dimethyl sulfoxide at −80°C" @default.
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- W2083647986 doi "https://doi.org/10.1016/s0011-2240(67)80154-8" @default.
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