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- W2083668672 abstract "phosphate buffer containing 44 mm-glucose and the spermatozoa washed twice and resuspended at the original concentration. Aliquots of the washed and unwashed spermatozoa were then placed in the CFDA and dialysed against the Krebs-Ringer and an otherwise identical hypotonic diluent (167 m-osmole/1) containing only 18m=.5mm-NaCl. Dialysis was continued for 6 hr at 35s=degC and the ICF/min measured with the impedance bridge and mechanical counter described by Martin & Wallace (1965). Aliquots of the unwashed and washed spermatozoa suspensions were also diluted 1:20 in Krebs-Ringer and hypotonic diluent and incubated for 5 hr at 35s=degC in specimen tubes. At 30-min intervals, motility was estimated on a warm stage at 37s=degC using the system of Emmens (1947) and the motility index was obtained by multiplying the motility score (0 to 4) by 4 (since quarter grades were frequently given) and summing over the incubation period. The results (Table 1) show no significant decrease in ICF when the spermatozoa were dialysed against the hypotonic diluent even after washing. The motility of spermatozoa diluted in hypotonic diluent was depressed, particularly after the cells had been washed. Since the ICF was maintained even after prior removal of high molecular weight substances from the dialysis sac by washing, the possible importance of rate of exposure to hypotonicity was next investigated (Table 2). Aliquots of ram semen were washed in Krebs-Ringer and hypotonie diluent and dialysed for 6 hr at 35° C in the CFDA or diluted 1:20 in both diluents and incubated at 35° C for 5 hr. As in the previous experiment, spermatozoa washed in KrebsRinger and dialysed against the hypotonie diluent maintained a high ICF," @default.
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- W2083668672 date "1969-03-01" @default.
- W2083668672 modified "2023-09-23" @default.
- W2083668672 title "OSMOTIC SHOCK OF RAM SPERMATOZOA" @default.
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- W2083668672 doi "https://doi.org/10.1530/jrf.0.0180375" @default.
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