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- W2083751570 abstract "A simple method was developed to isolate fumonisins B3 and B4 (FB3 and FB4) from cultures of a novel strain of Fusarium moniliforme which produces FB3 and FB4 but does not make FB1 or FB2. Undiluted extract was loaded onto a 10 g NH2 solid phase extraction cartridge, and the fumonisins were eluted with 5% acetic acid in methanol. The eluate was diluted with 1.5 vol of water and loaded onto a 10 g tC18 cartridge. The fumonisins were eluted with increasing amounts of acetonitrile in water. Complete separation was obtained between the FB3 (277 mg) and FB4 (62 mg) fractions. The fractions were analyzed for purity by three methods: fluorescence detection of the OPA derivatives, evaporative light-scattering detection of the underivatized fumonisins, and electrospray-MS. The fractions from the combined NH2/tC18 method contained more than 90% fumonisins. Recovery of the fumonisins from the extracts exceeded 95%. Keywords: Fumonisins; Fusarium moniliforme; corn; mycotoxins; isolation" @default.
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- W2083751570 date "1996-01-01" @default.
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- W2083751570 title "Rapid Purification of Fumonisins B<sub>3</sub> and B<sub>4</sub> with Solid Phase Extraction Columns" @default.
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- W2083751570 doi "https://doi.org/10.1021/jf960013p" @default.
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