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• W2083803630 abstract "Sites of toxicity of polycyclic aromatic hydrocarbons (PAHs) were examined to determine if inhibition of photosynthetic activity could be correlated to whole-organism toxicity. The inhibition of photosynthesis was observed by detecting the induction kinetics of endogenous chlorophyll a (Chl a) fluorescence. Anthracene (ANT) photooxidation products were applied to the aquatic higher plant Lemna gibba L. G-3 at concentrations ranging from 0.01 to 10 ppm. The impact on Chl a fluorescence was found to correlate with whole-organism toxicity for the 13 PAH compounds tested in this in vivo study. The mechanism of toxic action starts with inhibition of photosystem I (PSI) or the cytochrome-b6/f complex, followed by photooxidative damage to photosystem II (PSII). To study the effects of oxygenated ANTs on photosynthesis in vivo, the IC(50)s for F(V)/F(M) (PSII activity) and F(Q)/F(M) (activity downstream from PSII) were determined. The IC(50)s for a decrease of F(Q)/F(M) for all 13 chemicals were on average twofold lower than those for F(V)/F(M). F(V)/F(M) was found to be a measure of acute toxicity, whereas F(Q)/F(M) was found to be a measure of chronic toxicity. Thus, Chl a fluorescence by use of the whole organism was able to detect the impacts of photomodified ANT products and indicate a site of action for the chemicals." @default.
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• W2083803630 date "2002-01-01" @default.
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• W2083803630 title "Sites of toxicity of specific photooxidation products of anthracene to higher plants: Inhibition of photosynthetic activity and electron transport inLemna gibba L. G-3 (Duckweed)" @default.
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• W2083803630 doi "https://doi.org/10.1002/tox.10080" @default.
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