FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2083822728> ?p ?o ?g. }

• W2083822728 endingPage "3844" @default.
• W2083822728 startingPage "3835" @default.
• W2083822728 abstract "O-GlcNAcase is a family 84 β-N-acetylglucosaminidase catalyzing the hydrolytic cleavage of β-O-linked 2-acetamido-2-deoxy-d-glycopyranose (O-GlcNAc) from serine and threonine residues of posttranslationally modified proteins. O-GlcNAcases use a double-displacement mechanism involving formation and breakdown of a transient bicyclic oxazoline intermediate. The key catalytic residues of any family 84 enzyme facilitating this reaction, however, are unknown. Two mutants of human O-GlcNAcase, D174A and D175A, were generated since these residues are highly conserved among family 84 glycoside hydrolases. Structure−reactivity studies of the D174A mutant enzyme reveals severely impaired catalytic activity across a broad range of substrates alongside a pH−activity profile consistent with deletion of a key catalytic residue. The D175A mutant enzyme shows a significant decrease in catalytic efficiency with substrates bearing poor leaving groups (up to 3000-fold), while for substates bearing good leading groups the difference is much smaller (7-fold). This mutant enzyme also cleaves thioglycosides with essentially the same catalytic efficiency as the wild-type enzyme. As well, addition of azide as an exogenous nucleophile increases the activity of this enzyme toward a substrate bearing an excellent leaving group. Together, these results allow unambiguous assignment of Asp174 as the residue that polarizes the 2-acetamido group for attack on the anomeric center and Asp175 as the residue that functions as the general acid/base catalyst. Therefore, the family 84 glycoside hydrolases use a DD catalytic pair to effect catalysis." @default.
• W2083822728 created "2016-06-24" @default.
• W2083822728 creator A5008486770 @default.
• W2083822728 creator A5066227932 @default.
• W2083822728 creator A5078248083 @default.
• W2083822728 creator A5079183034 @default.
• W2083822728 creator A5088501340 @default.
• W2083822728 date "2006-02-24" @default.
• W2083822728 modified "2023-10-06" @default.
• W2083822728 title "Identification of Asp¹⁷⁴ and Asp¹⁷⁵ as the Key Catalytic Residues of Human <i>O</i>-GlcNAcase by Functional Analysis of Site-Directed Mutants" @default.
• W2083822728 cites W1830745998 @default.
• W2083822728 cites W1969939865 @default.
• W2083822728 cites W1989430502 @default.
• W2083822728 cites W1990762118 @default.
• W2083822728 cites W1990809364 @default.
• W2083822728 cites W1992808956 @default.
• W2083822728 cites W2019767057 @default.
• W2083822728 cites W2024882816 @default.
• W2083822728 cites W2045625530 @default.
• W2083822728 cites W2052954694 @default.
• W2083822728 cites W2086621993 @default.
• W2083822728 cites W2108956277 @default.
• W2083822728 cites W2112185869 @default.
• W2083822728 cites W2119033055 @default.
• W2083822728 cites W2121513967 @default.
• W2083822728 cites W2136260330 @default.
• W2083822728 cites W2156864264 @default.
• W2083822728 cites W2167602049 @default.
• W2083822728 cites W2220462402 @default.
• W2083822728 doi "https://doi.org/10.1021/bi052370b" @default.
• W2083822728 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16533067" @default.
• W2083822728 hasPublicationYear "2006" @default.
• W2083822728 type Work @default.
• W2083822728 sameAs 2083822728 @default.
• W2083822728 citedByCount "110" @default.
• W2083822728 countsByYear W20838227282012 @default.
• W2083822728 countsByYear W20838227282013 @default.
• W2083822728 countsByYear W20838227282014 @default.
• W2083822728 countsByYear W20838227282015 @default.
• W2083822728 countsByYear W20838227282016 @default.
• W2083822728 countsByYear W20838227282017 @default.
• W2083822728 countsByYear W20838227282018 @default.
• W2083822728 countsByYear W20838227282019 @default.
• W2083822728 countsByYear W20838227282020 @default.
• W2083822728 countsByYear W20838227282021 @default.
• W2083822728 countsByYear W20838227282022 @default.
• W2083822728 countsByYear W20838227282023 @default.
• W2083822728 crossrefType "journal-article" @default.
• W2083822728 hasAuthorship W2083822728A5008486770 @default.
• W2083822728 hasAuthorship W2083822728A5066227932 @default.
• W2083822728 hasAuthorship W2083822728A5078248083 @default.
• W2083822728 hasAuthorship W2083822728A5079183034 @default.
• W2083822728 hasAuthorship W2083822728A5088501340 @default.
• W2083822728 hasBestOaLocation W20838227282 @default.
• W2083822728 hasConcept C104317684 @default.
• W2083822728 hasConcept C143065580 @default.
• W2083822728 hasConcept C147816474 @default.
• W2083822728 hasConcept C160964918 @default.
• W2083822728 hasConcept C161790260 @default.
• W2083822728 hasConcept C181199279 @default.
• W2083822728 hasConcept C185592680 @default.
• W2083822728 hasConcept C2775880066 @default.
• W2083822728 hasConcept C2776414213 @default.
• W2083822728 hasConcept C2781338088 @default.
• W2083822728 hasConcept C41183919 @default.
• W2083822728 hasConcept C55493867 @default.
• W2083822728 hasConcept C69118441 @default.
• W2083822728 hasConcept C71240020 @default.
• W2083822728 hasConceptScore W2083822728C104317684 @default.
• W2083822728 hasConceptScore W2083822728C143065580 @default.
• W2083822728 hasConceptScore W2083822728C147816474 @default.
• W2083822728 hasConceptScore W2083822728C160964918 @default.
• W2083822728 hasConceptScore W2083822728C161790260 @default.
• W2083822728 hasConceptScore W2083822728C181199279 @default.
• W2083822728 hasConceptScore W2083822728C185592680 @default.
• W2083822728 hasConceptScore W2083822728C2775880066 @default.
• W2083822728 hasConceptScore W2083822728C2776414213 @default.
• W2083822728 hasConceptScore W2083822728C2781338088 @default.
• W2083822728 hasConceptScore W2083822728C41183919 @default.
• W2083822728 hasConceptScore W2083822728C55493867 @default.
• W2083822728 hasConceptScore W2083822728C69118441 @default.
• W2083822728 hasConceptScore W2083822728C71240020 @default.
• W2083822728 hasIssue "11" @default.
• W2083822728 hasLocation W20838227281 @default.
• W2083822728 hasLocation W20838227282 @default.
• W2083822728 hasLocation W20838227283 @default.
• W2083822728 hasOpenAccess W2083822728 @default.
• W2083822728 hasPrimaryLocation W20838227281 @default.
• W2083822728 hasRelatedWork W1498022526 @default.
• W2083822728 hasRelatedWork W1550634984 @default.
• W2083822728 hasRelatedWork W1964529071 @default.
• W2083822728 hasRelatedWork W1990598130 @default.
• W2083822728 hasRelatedWork W2002381976 @default.
• W2083822728 hasRelatedWork W2005874283 @default.
• W2083822728 hasRelatedWork W2052915805 @default.
• W2083822728 hasRelatedWork W2056269112 @default.
• W2083822728 hasRelatedWork W2067933933 @default.
• W2083822728 hasRelatedWork W2330714631 @default.

• next