Matches in SemOpenAlex for { <https://semopenalex.org/work/W2083889114> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W2083889114 abstract "Our initial in vitro (HL-60 cells) and in vivo (B16-F10 murine) studies showed nanosecond pulsed electric fields (nsPEFs) caused intracellular changes and melanoma involution, respectively. We wanted to describe the morphologic changes in cell ultrastructure and investigate the mechanism for change due to nsPEFs in B16-F10 melanoma tumors in SKH-1 mice. We injected B16-F10 cells into 120 female SKH-1 mice to derive melanoma tumors. After multiple nsPEF treatments (40 kV/cm field strength; 5 ns rise time; 300 ns duration), morphologic changes of melanoma size, shape, tumor nesting pattern, blood vessel structure and cell ultrastructure were observed. Nuclear changes were recorded with light-and transmission electron-microscopy (TEM). Apoptosis in situ was detected by several immunohistochemistry (IHC) methods: (1) terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL); (2) caspase 3, 6 and 7; and (3) histone H2AX. After multiple nsPEF treatments the melanoma blood supply was disrupted and melanomas shrank an average of 90% within two weeks. TEM showed increased rough endoplasmic reticulum and condensed dark-staining nucleoli; TUNEL presented fluorescent increase. Together these suggest post-treatment apoptotic activity, which was concurrently allied with increased caspase 3, 6 and 7, and histone H2AX fluorescence (DNA damage) after pulsing. Our study shows that blood flow and apoptotic changes play a central role in the biological effects caused by nsPEFs. Therefore nsPEFs may have application potential in cancer therapy, gene regulation and biophysical research by non-invasively disrupting intracellular components and inducing apoptosis in malignant tumors." @default.
- W2083889114 created "2016-06-24" @default.
- W2083889114 creator A5014266369 @default.
- W2083889114 creator A5014727739 @default.
- W2083889114 creator A5020943599 @default.
- W2083889114 creator A5025283381 @default.
- W2083889114 creator A5045768131 @default.
- W2083889114 creator A5047618158 @default.
- W2083889114 creator A5070920697 @default.
- W2083889114 creator A5084943724 @default.
- W2083889114 creator A5085878372 @default.
- W2083889114 date "2007-09-01" @default.
- W2083889114 modified "2023-09-23" @default.
- W2083889114 title "Melanoma Morphology Change & Apoptosis Induced by Multiple Nanosecond Pulsed Electric Fields" @default.
- W2083889114 cites W1963695337 @default.
- W2083889114 cites W1972708469 @default.
- W2083889114 cites W1984457499 @default.
- W2083889114 cites W1992465102 @default.
- W2083889114 cites W2010331522 @default.
- W2083889114 cites W2020951873 @default.
- W2083889114 cites W2031142337 @default.
- W2083889114 cites W2043485308 @default.
- W2083889114 cites W2055662392 @default.
- W2083889114 cites W2056221094 @default.
- W2083889114 cites W2087765747 @default.
- W2083889114 cites W2104408549 @default.
- W2083889114 cites W2109581113 @default.
- W2083889114 cites W2113617078 @default.
- W2083889114 cites W2114144028 @default.
- W2083889114 cites W2146446771 @default.
- W2083889114 doi "https://doi.org/10.1109/iceaa.2007.4387486" @default.
- W2083889114 hasPublicationYear "2007" @default.
- W2083889114 type Work @default.
- W2083889114 sameAs 2083889114 @default.
- W2083889114 citedByCount "2" @default.
- W2083889114 countsByYear W20838891142018 @default.
- W2083889114 countsByYear W20838891142019 @default.
- W2083889114 crossrefType "proceedings-article" @default.
- W2083889114 hasAuthorship W2083889114A5014266369 @default.
- W2083889114 hasAuthorship W2083889114A5014727739 @default.
- W2083889114 hasAuthorship W2083889114A5020943599 @default.
- W2083889114 hasAuthorship W2083889114A5025283381 @default.
- W2083889114 hasAuthorship W2083889114A5045768131 @default.
- W2083889114 hasAuthorship W2083889114A5047618158 @default.
- W2083889114 hasAuthorship W2083889114A5070920697 @default.
- W2083889114 hasAuthorship W2083889114A5084943724 @default.
- W2083889114 hasAuthorship W2083889114A5085878372 @default.
- W2083889114 hasConcept C142724271 @default.
- W2083889114 hasConcept C153911025 @default.
- W2083889114 hasConcept C154113507 @default.
- W2083889114 hasConcept C185592680 @default.
- W2083889114 hasConcept C190283241 @default.
- W2083889114 hasConcept C196795494 @default.
- W2083889114 hasConcept C2777658100 @default.
- W2083889114 hasConcept C502942594 @default.
- W2083889114 hasConcept C55493867 @default.
- W2083889114 hasConcept C71924100 @default.
- W2083889114 hasConcept C86803240 @default.
- W2083889114 hasConcept C95444343 @default.
- W2083889114 hasConceptScore W2083889114C142724271 @default.
- W2083889114 hasConceptScore W2083889114C153911025 @default.
- W2083889114 hasConceptScore W2083889114C154113507 @default.
- W2083889114 hasConceptScore W2083889114C185592680 @default.
- W2083889114 hasConceptScore W2083889114C190283241 @default.
- W2083889114 hasConceptScore W2083889114C196795494 @default.
- W2083889114 hasConceptScore W2083889114C2777658100 @default.
- W2083889114 hasConceptScore W2083889114C502942594 @default.
- W2083889114 hasConceptScore W2083889114C55493867 @default.
- W2083889114 hasConceptScore W2083889114C71924100 @default.
- W2083889114 hasConceptScore W2083889114C86803240 @default.
- W2083889114 hasConceptScore W2083889114C95444343 @default.
- W2083889114 hasLocation W20838891141 @default.
- W2083889114 hasOpenAccess W2083889114 @default.
- W2083889114 hasPrimaryLocation W20838891141 @default.
- W2083889114 hasRelatedWork W1988757932 @default.
- W2083889114 hasRelatedWork W2056570618 @default.
- W2083889114 hasRelatedWork W2358167431 @default.
- W2083889114 hasRelatedWork W2366061230 @default.
- W2083889114 hasRelatedWork W2381162594 @default.
- W2083889114 hasRelatedWork W2381820491 @default.
- W2083889114 hasRelatedWork W2388367751 @default.
- W2083889114 hasRelatedWork W2391858692 @default.
- W2083889114 hasRelatedWork W3143982046 @default.
- W2083889114 hasRelatedWork W788896063 @default.
- W2083889114 isParatext "false" @default.
- W2083889114 isRetracted "false" @default.
- W2083889114 magId "2083889114" @default.
- W2083889114 workType "article" @default.