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- W2083896317 abstract "Glycine soja Sieb. et Zucc. (a wild species of soybean) protoplasts were isolated from immature cotyledons and initially cultured in a modified K8P medium supplemented with 0.2 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D), 1 mg/l naphthalene acetic acid (NAA) and 0.4 mg/l benzyl adenine (BA). The protoplasts started to divide after 3 days of culture. The division frequency of protoplast-derived cells counted at 12 days reached 36.8%. After 6 weeks, cell colonies formed and transfer to gelrite-solidified K8 cell medium with 0.1–0.2 mg/l NAA and 0.5–2 mg/l BA supported further proliferation and callus growth. When calli of 2–3 mm in size were subcultured on MSB growth medium with 0.2 mg/l NAA and 0.5 mg/l BA, approximately 15% of them developed into compact and nodular calli. Shoot buds were regenerated on the surface of the nodular calli with a frequency of 25%, when the calli were placed on MSB medium with 0.1 mg/l indole-3-acetic acid (IAA) and 0.2–0.5 mg/l BA. Whole plants were regenerated upon transfer of 3–4 cm shoots to 12 Murashige and Skoog (MS) medium with 0.2 mg/l indole butyric acid (IBA). Twenty-four complete plants have been obtained and seven of them transplanted into pots are growing well in the phytotron." @default.
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- W2083896317 date "1990-01-01" @default.
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- W2083896317 title "Plant regeneration from protoplasts of immature cotyledons of Glycine soja Sieb. et Zucc." @default.
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- W2083896317 doi "https://doi.org/10.1016/0168-9452(90)90037-o" @default.
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