FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2083992079> ?p ?o ?g. }

• W2083992079 endingPage "447" @default.
• W2083992079 startingPage "439" @default.
• W2083992079 abstract "The activation of caspase-3 represents a critical step in the pathways leading to the biochemical and morphological changes that underlie apoptosis. Upon induction of apoptosis, the large (p17) and small (p12) subunits, comprising active caspase-3, are generated via proteolytic processing of a latent proenzyme dimer. Two copies of each individual subunit are generated to form an active heterotetramer. The tetrameric form of caspase-3 cleaves specific protein substrates within the cell, thereby producing the apoptotic phenotype. In contrast to the proenzyme, once activated in HeLa cells, caspase-3 is difficult to detect due to its rapid degradation. Interestingly, however, enzyme stability and therefore detection of active caspase-3 by immunoblot analysis can be restored by treatment of cells with a peptide-based caspase-3 selective inhibitor, suggesting that the active form can be stabilized through protein-inhibitor interaction. The heteromeric active enzyme complex is necessary for its stabilization by inhibitors, as expression of the large subunit alone is not stabilized by the presence of inhibitors. Our results show for the first time, that synthetic caspase inhibitors not only block caspase activity, but may also increase the stability of otherwise rapidly degraded mature caspase complexes. Consistent with these findings, experiments with a catalytically inactive mutant of caspase-3 show that rapid turnover is dependent on the activity of the mature enzyme. Furthermore, turnover of otherwise stable active site mutants of capase-3 is rescued by the presence of the active enzyme suggesting that turnover can be mediated in trans." @default.
• W2083992079 created "2016-06-24" @default.
• W2083992079 creator A5002601703 @default.
• W2083992079 creator A5018266345 @default.
• W2083992079 creator A5042528414 @default.
• W2083992079 creator A5048595906 @default.
• W2083992079 creator A5058383262 @default.
• W2083992079 creator A5068662836 @default.
• W2083992079 creator A5088611150 @default.
• W2083992079 date "2004-01-09" @default.
• W2083992079 modified "2023-10-16" @default.
• W2083992079 title "Catalytic activity of caspase-3 is required for its degradation: stabilization of the active complex by synthetic inhibitors" @default.
• W2083992079 cites W1544768334 @default.
• W2083992079 cites W1557686543 @default.
• W2083992079 cites W1589415602 @default.
• W2083992079 cites W1616649922 @default.
• W2083992079 cites W1662899239 @default.
• W2083992079 cites W1745634660 @default.
• W2083992079 cites W1912063668 @default.
• W2083992079 cites W1966176339 @default.
• W2083992079 cites W1967912613 @default.
• W2083992079 cites W1987785848 @default.
• W2083992079 cites W1988969017 @default.
• W2083992079 cites W1990683719 @default.
• W2083992079 cites W1991865867 @default.
• W2083992079 cites W2000407232 @default.
• W2083992079 cites W2007939817 @default.
• W2083992079 cites W2012173926 @default.
• W2083992079 cites W2024437197 @default.
• W2083992079 cites W2036568037 @default.
• W2083992079 cites W2039597975 @default.
• W2083992079 cites W2054260525 @default.
• W2083992079 cites W2057292793 @default.
• W2083992079 cites W2061497413 @default.
• W2083992079 cites W2064127449 @default.
• W2083992079 cites W2077628780 @default.
• W2083992079 cites W2097531829 @default.
• W2083992079 cites W2114344465 @default.
• W2083992079 cites W2135662322 @default.
• W2083992079 cites W2138144052 @default.
• W2083992079 cites W2151501496 @default.
• W2083992079 cites W2154554320 @default.
• W2083992079 cites W2154571107 @default.
• W2083992079 cites W2167490100 @default.
• W2083992079 cites W2169205569 @default.
• W2083992079 doi "https://doi.org/10.1038/sj.cdd.4401360" @default.
• W2083992079 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14713960" @default.
• W2083992079 hasPublicationYear "2004" @default.
• W2083992079 type Work @default.
• W2083992079 sameAs 2083992079 @default.
• W2083992079 citedByCount "82" @default.
• W2083992079 countsByYear W20839920792012 @default.
• W2083992079 countsByYear W20839920792013 @default.
• W2083992079 countsByYear W20839920792014 @default.
• W2083992079 countsByYear W20839920792015 @default.
• W2083992079 countsByYear W20839920792016 @default.
• W2083992079 countsByYear W20839920792017 @default.
• W2083992079 countsByYear W20839920792018 @default.
• W2083992079 countsByYear W20839920792019 @default.
• W2083992079 countsByYear W20839920792020 @default.
• W2083992079 countsByYear W20839920792021 @default.
• W2083992079 countsByYear W20839920792022 @default.
• W2083992079 countsByYear W20839920792023 @default.
• W2083992079 crossrefType "journal-article" @default.
• W2083992079 hasAuthorship W2083992079A5002601703 @default.
• W2083992079 hasAuthorship W2083992079A5018266345 @default.
• W2083992079 hasAuthorship W2083992079A5042528414 @default.
• W2083992079 hasAuthorship W2083992079A5048595906 @default.
• W2083992079 hasAuthorship W2083992079A5058383262 @default.
• W2083992079 hasAuthorship W2083992079A5068662836 @default.
• W2083992079 hasAuthorship W2083992079A5088611150 @default.
• W2083992079 hasBestOaLocation W20839920791 @default.
• W2083992079 hasConcept C104292427 @default.
• W2083992079 hasConcept C104317684 @default.
• W2083992079 hasConcept C125209161 @default.
• W2083992079 hasConcept C143065580 @default.
• W2083992079 hasConcept C181199279 @default.
• W2083992079 hasConcept C185592680 @default.
• W2083992079 hasConcept C190283241 @default.
• W2083992079 hasConcept C2780126713 @default.
• W2083992079 hasConcept C2781307694 @default.
• W2083992079 hasConcept C31573885 @default.
• W2083992079 hasConcept C41183919 @default.
• W2083992079 hasConcept C55493867 @default.
• W2083992079 hasConcept C86803240 @default.
• W2083992079 hasConcept C95444343 @default.
• W2083992079 hasConcept C98424977 @default.
• W2083992079 hasConceptScore W2083992079C104292427 @default.
• W2083992079 hasConceptScore W2083992079C104317684 @default.
• W2083992079 hasConceptScore W2083992079C125209161 @default.
• W2083992079 hasConceptScore W2083992079C143065580 @default.
• W2083992079 hasConceptScore W2083992079C181199279 @default.
• W2083992079 hasConceptScore W2083992079C185592680 @default.
• W2083992079 hasConceptScore W2083992079C190283241 @default.
• W2083992079 hasConceptScore W2083992079C2780126713 @default.
• W2083992079 hasConceptScore W2083992079C2781307694 @default.
• W2083992079 hasConceptScore W2083992079C31573885 @default.
• W2083992079 hasConceptScore W2083992079C41183919 @default.

• next