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- W2084037101 abstract "The interaction of an important acridine dye, proflavine hydrochloride, with double stranded DNA was investigated using isothermal titration calorimetry and differential scanning calorimetry. The equilibrium constant for the binding reaction was calculated to be (1.60 ± 0.04) · 105 · M−1 at T = 298.15 K. The binding of proflavine hydrochloride to DNA was favored by both negative enthalpy and positive entropy contributions to the Gibbs energy. The equilibrium constant for the binding reaction decreased with increasing temperature. The standard molar enthalpy change became increasingly negative while the standard molar entropy change became less positive with rise in temperature. However, the standard molar Gibbs free energy change varied marginally suggesting the occurrence of enthalpy–entropy compensation phenomenon. The binding reaction was dominated by non-polyelectrolytic forces which remained virtually unchanged at all the salt concentrations studied. The binding also significantly increased the thermal stability of DNA against thermal denaturation." @default.
- W2084037101 created "2016-06-24" @default.
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- W2084037101 date "2015-08-01" @default.
- W2084037101 modified "2023-10-18" @default.
- W2084037101 title "Thermodynamic characterization of proflavine–DNA binding through microcalorimetric studies" @default.
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- W2084037101 doi "https://doi.org/10.1016/j.jct.2015.03.009" @default.
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