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• W2084047478 abstract "The protein predicted to be encoded by varicella-zoster virus (VZV) gene 61 exhibits limited amino acid sequence similarity to the herpes simplex virus type 1 nuclear phosphoprotein Vmw110, which functions as a transcriptional activator. The gene 61 protein was expressed in its entirety, or as an amino- or carboxy-terminal fragment in Escherichia coli and vaccinia virus recombinants, and monospecific rabbit antisera were raised against an E. coli fusion between beta-galactosidase and the majority of the gene 61 protein. Use of the antisera showed that the gene 61 protein is present in VZV-infected cell nuclei as a heterogeneous phospho-protein of Mr62K to 65K. Phosphorylation occurs in the amino- and, to a lesser extent, carboxy-terminal portions of the protein. The carboxy-terminal region directs transport of the protein to the nucleus, whereas the amino-terminal region, which contains a potential zinc-binding domain, is responsible for a punctate distribution. Preliminary mapping data indicated that gene 61 is transcribed as a 1.8 kb mRNA which initiates about 65 bp upstream from the translation initiation codon, at a position located appropriately with respect to potential regulatory elements." @default.
• W2084047478 created "2016-06-24" @default.
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• W2084047478 date "1992-03-01" @default.
• W2084047478 modified "2023-09-24" @default.
• W2084047478 title "Characterization of the varicella-zoster virus gene 61 protein" @default.
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• W2084047478 doi "https://doi.org/10.1099/0022-1317-73-3-521" @default.
• W2084047478 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1312115" @default.
• W2084047478 hasPublicationYear "1992" @default.
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