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- W2084067422 abstract "In this study, a recombinant cellobiose 2-epimerase (GenBank accession number, YP_006392930.1) was characterized from a thermophilic bacterium, Thermoanaerobacterium saccharolyticum JW/SL-YS485. The enzyme was metal independent, showed maximal epimerization activity at pH 7.0 and 60 °C, and displayed 29.8, 15.48, and 13.5 U mg−1 for mannobiose, cellobiose, and lactose under optimum conditions, respectively. It exhibited promising thermostability under incubation below 60 °C. The Km, turnover number (kcat), and catalytic efficiency (kcat/Km) for lactose were 124.7 mM, 30.9 s−1, and 0.248 mM−1 s−1, respectively. At pH 7.0 and 60 °C, 50 mM epilactose was produced from 200 mM lactose by a 0.6 μM of enzyme concentration after reaction for 4 h." @default.
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- W2084067422 date "2015-06-01" @default.
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- W2084067422 title "Characterization of an epilactose-producing cellobiose 2-epimerase from Thermoanaerobacterium saccharolyticum" @default.
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- W2084067422 doi "https://doi.org/10.1016/j.molcatb.2015.03.005" @default.
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