FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2084077617> ?p ?o ?g. }

• W2084077617 endingPage "9113" @default.
• W2084077617 startingPage "9088" @default.
• W2084077617 abstract "Recognition of nucleic acids is important for our understanding of nucleic acid structure as well as for our understanding of nucleic acid-protein interactions. In addition to the direct readout mechanisms of nucleic acids such as H-bonding, shape recognition of nucleic acids is being increasingly recognized as playing an equally important role in DNA recognition. Competition dialysis, UV, flourescent intercalator displacement (FID), computational docking, and calorimetry studies were conducted to study the interaction of neomycin with a variety of nucleic acid conformations (shapes). At pH 5.5, the results suggest the following. (1) Neomycin binds three RNA structures [16S A site rRNA, poly(rA)·poly(rA), and poly(rA)·poly(rU)] with high affinities (K(a) ~ 10(7) M(-1)). (2) The binding of neomycin to A-form GC-rich oligomer d(A(2)G(15)C(15)T(2))(2) has an affinity comparable to those of RNA structures. (3) The binding of neomycin to DNA·RNA hybrids shows a 3-fold variance that can be attributed to their structural differences [for poly(dA)·poly(rU), K(a) = 9.4 × 10(6) M(-1), and for poly(rA)·poly(dT), K(a) = 3.1 × 10(6) M(-1)]. (4) The interaction of neomycin with DNA triplex poly(dA)·2poly(dT) yields a binding affinity (K(a)) of 2.4 × 10(5) M(-1). (5) Poly(dA-dT)(2) shows the lowest association constant for all nucleic acids studied (K(a) < 10(5)). (6) Neomycin binds to G-quadruplexes with K(a) values of ~10(4)-10(5) M(-1). (7) Computational studies show that the decrease in major groove width in the B to A transition correlates with increasing neomycin affinity. Neomycin's affinity for various nucleic acid structures can be ranked as follows: RNAs and GC-rich d(A(2)G(15)C(15)T(2))(2) structures > poly(dA)·poly(rU) > poly(rA)·poly(dT) > T·A-T triplex, G-quadruplex, B-form AT-rich, or GC-rich DNA sequences. The results illustrate the first example of a small molecule-based shape readout of different nucleic acid conformations." @default.
• W2084077617 created "2016-06-24" @default.
• W2084077617 creator A5002532676 @default.
• W2084077617 creator A5047542745 @default.
• W2084077617 creator A5062332920 @default.
• W2084077617 creator A5070623364 @default.
• W2084077617 creator A5075517061 @default.
• W2084077617 creator A5078824489 @default.
• W2084077617 date "2011-10-03" @default.
• W2084077617 modified "2023-09-30" @default.
• W2084077617 title "Thermodynamics of Nucleic Acid “Shape Readout” by an Aminosugar" @default.
• W2084077617 cites W1497009944 @default.
• W2084077617 cites W1528818827 @default.
• W2084077617 cites W1965127482 @default.
• W2084077617 cites W1965252702 @default.
• W2084077617 cites W1967538502 @default.
• W2084077617 cites W1970030024 @default.
• W2084077617 cites W1970033940 @default.
• W2084077617 cites W1971481046 @default.
• W2084077617 cites W1972353318 @default.
• W2084077617 cites W1972927281 @default.
• W2084077617 cites W1974197041 @default.
• W2084077617 cites W1977263723 @default.
• W2084077617 cites W1979887857 @default.
• W2084077617 cites W1983023891 @default.
• W2084077617 cites W1991594629 @default.
• W2084077617 cites W1992879866 @default.
• W2084077617 cites W1993394468 @default.
• W2084077617 cites W1994675124 @default.
• W2084077617 cites W1996420060 @default.
• W2084077617 cites W1996815852 @default.
• W2084077617 cites W1997237296 @default.
• W2084077617 cites W2001701885 @default.
• W2084077617 cites W2002763632 @default.
• W2084077617 cites W2004337049 @default.
• W2084077617 cites W2004906161 @default.
• W2084077617 cites W2006479906 @default.
• W2084077617 cites W2007812774 @default.
• W2084077617 cites W2007946692 @default.
• W2084077617 cites W2012627860 @default.
• W2084077617 cites W2012997805 @default.
• W2084077617 cites W2015162132 @default.
• W2084077617 cites W2015611931 @default.
• W2084077617 cites W2016446921 @default.
• W2084077617 cites W2016636999 @default.
• W2084077617 cites W2020164435 @default.
• W2084077617 cites W2020375331 @default.
• W2084077617 cites W2023274087 @default.
• W2084077617 cites W2023638011 @default.
• W2084077617 cites W2025777803 @default.
• W2084077617 cites W2025811126 @default.
• W2084077617 cites W2028214064 @default.
• W2084077617 cites W2031750380 @default.
• W2084077617 cites W2035738941 @default.
• W2084077617 cites W2036150293 @default.
• W2084077617 cites W2037865206 @default.
• W2084077617 cites W2041175802 @default.
• W2084077617 cites W2042712469 @default.
• W2084077617 cites W2044791372 @default.
• W2084077617 cites W2046723537 @default.
• W2084077617 cites W2047209080 @default.
• W2084077617 cites W2049502419 @default.
• W2084077617 cites W2049884513 @default.
• W2084077617 cites W2050628347 @default.
• W2084077617 cites W2050939941 @default.
• W2084077617 cites W2052127381 @default.
• W2084077617 cites W2053166926 @default.
• W2084077617 cites W2054011799 @default.
• W2084077617 cites W2056882517 @default.
• W2084077617 cites W2057541073 @default.
• W2084077617 cites W2058598015 @default.
• W2084077617 cites W2060389234 @default.
• W2084077617 cites W2066355347 @default.
• W2084077617 cites W2066801081 @default.
• W2084077617 cites W2069002950 @default.
• W2084077617 cites W2072170792 @default.
• W2084077617 cites W2073787422 @default.
• W2084077617 cites W2075005664 @default.
• W2084077617 cites W2075529035 @default.
• W2084077617 cites W2077297926 @default.
• W2084077617 cites W2077848527 @default.
• W2084077617 cites W2079549728 @default.
• W2084077617 cites W2079961585 @default.
• W2084077617 cites W2079991869 @default.
• W2084077617 cites W2081083186 @default.
• W2084077617 cites W2084291832 @default.
• W2084077617 cites W2090545729 @default.
• W2084077617 cites W2093687460 @default.
• W2084077617 cites W2104400477 @default.
• W2084077617 cites W2107740970 @default.
• W2084077617 cites W2109359405 @default.
• W2084077617 cites W2110534779 @default.
• W2084077617 cites W2111332057 @default.
• W2084077617 cites W2112939001 @default.
• W2084077617 cites W2115639078 @default.
• W2084077617 cites W2119970960 @default.
• W2084077617 cites W2123951763 @default.
• W2084077617 cites W2128744040 @default.

• next