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- W2084098984 abstract "Three rabbits immunized with hen egg-white lysozyme (three injections in complete Freund's adjuvant) were bled weekly for a period of 4 months. Each individual antiserum was tested for the presence of antibodies specific towards both native lysozyme and two distinct antigenic determinants of the molecule, comprising the “loop” peptide (residues 60-83 containing one disulfide bond) and a fragment containing the amino and carboxy termini of lysozyme. Variations were observed in the amounts and the time of appearance of antibodies with different specificities. The first demonstrable antibodies, 10 days after the onset of immunization, were antibodies directed towards intact lysozyme. At this time, no antibodies to the two isolated antigenic fragments of the molecule could be detected. Antibodies specific for these two determinants appeared at much later bleedings 2-3 months after immunization. Although the general pattern of the induction of antibodies with different specificities was the same in all three rabbits, individual differences were never-theless observed among the three animals used in this study." @default.
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- W2084098984 date "1971-01-01" @default.
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- W2084098984 title "Sequential appearance of antibodies directed against different antigenic determinants of hen egg-white lysozyme" @default.
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- W2084098984 doi "https://doi.org/10.1002/eji.1830010307" @default.
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