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• W2084133052 abstract "Human lysosomal α-L-iduronidase, whose deficiency causes mucopolysaccharidosis type I, was crystallized using sodium/potassium tartrate and polyethylene glycol 3350 as a precipitant. Using synchrotron radiation, a native data set was collected from a single crystal at 100 K to 2.3 Å resolution. The crystal belonged to space group R 3 with unit-cell dimensions of a = b = 259.22, c = 71.83 Å. To obtain the phase information, mercury-derivative crystals were prepared and a single-wavelength anomalous dispersion (SAD) data set was collected at the Hg peak wavelength. Phase calculation with the single isomorphous replacement with anomalous scattering (SIRAS) method successfully yielded an interpretable electron-density map." @default.
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• W2084133052 date "2012-10-30" @default.
• W2084133052 modified "2023-09-27" @default.
• W2084133052 title "Crystallization, X-ray diffraction analysis and SIRAS phasing of human α-L-iduronidase" @default.
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• W2084133052 doi "https://doi.org/10.1107/s1744309112040432" @default.
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