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- W2084160835 abstract "The sensitivity of the oxygen uptake of soybean embryonic axes and of isolated mitochondria to the inhibitors salicylhydroxamic acid (SHAM), di-tert butyl hydroquinone (DTBQ), potassium cyanide and disulfiram, was used to determine the contribution of cytochrome oxidase, alternative oxidase and lipoxygenase to the total oxygen consumption of soybean embryonic axes over the period of 2–30 h of inhibition. Cyanide-sensitive oxygen uptake (ascribed to cytochrome oxidase) increased from 2.0 to 4.9 μl O2/h per axis. SHAM-sensitive minus DTBQ-sensitive oxygen uptake or disulfiram-sensitive respiration (ascribed to alternative oxidase) accounted for 0.7 to 0.9 μl O2/h per axis. DTBQ-sensitive oxygen uptake (ascribed to lipoxygenase) increased from 0.26 to 2.1 μl O2/h per axis. The activity of the alternative pathway of isolated mitochondria did not change (2.9 ± 0.1 nmol O2/min per mg protein) in embryonic axes that were inbibed for 2 to 14 h. The activity of the cytochrome pathway in isolated mitochondria and in the whole homogenate increased by a factor of about 4 upon imbibition from 2 to 30 h. Added cytochrome c markedly increased (123%–170%) the oxygen uptake of mitochondria from embryonic axes that were imbibed for 2–14 h and had a much lower effect (14%) on the oxygen uptake of mitochondria from embryonic axes imbibed for 36 h." @default.
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- W2084160835 date "1987-01-01" @default.
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- W2084160835 title "Oxidative activities in soybean embryonic axes during germination" @default.
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- W2084160835 doi "https://doi.org/10.1016/0168-9452(87)90102-6" @default.
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