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• W2084205541 endingPage "382" @default.
• W2084205541 startingPage "376" @default.
• W2084205541 abstract "Shrimp and crab shell powder (SCSP), prepared by treating shellfish processing waste with boiling and crushing, was used as a substrate for isolating chitinolytic microorganisms. Three potential strains (E1, J1, and J1-1) were isolated and identified as Bacillus cereus, B. alvei, and B. sphaericus, respectively. Three extracellular chitinases (FB1, FB2, and FB3) were purified from the culture supernatants of Bacillus cereus E1, B. alvei J1, and B. sphaericus J1-1, respectively. The molecular weights of FB1, FB2, and FB3 were 71,000, 71,000, and 65,000, respectively, by SDS-PAGE. The pIs for FB1, FB2, and FB3 were 7.1, 7.2, and 7.4, respectively. The optimum pH, optimum temperature, pH stability, and thermal stability of FB1 were pH 9, 50 degrees C, pH 7 to 10, and 70 degrees C; those of FB2 were pH 9, 60 degrees C, pH 5 to 9, and 70 degrees C; and those of FB3 were pH 7, 50 degrees C, pH 5 to 9, and 60 degrees C. The activities of all enzymes were strongly inhibited by Hg(2+) and completely inhibited by glutathione, dithiothreitol, and 2-mercaptoethanol." @default.
• W2084205541 created "2016-06-24" @default.
• W2084205541 creator A5007027446 @default.
• W2084205541 creator A5071754735 @default.
• W2084205541 date "2001-03-01" @default.
• W2084205541 modified "2023-10-12" @default.
• W2084205541 title "Microbial reclamation of shellfish wastes for the production of chitinases" @default.
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• W2084205541 doi "https://doi.org/10.1016/s0141-0229(00)00325-2" @default.
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• W2084205541 hasPublicationYear "2001" @default.
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