Matches in SemOpenAlex for { <https://semopenalex.org/work/W2084283669> ?p ?o ?g. }
- W2084283669 endingPage "e78936" @default.
- W2084283669 startingPage "e78936" @default.
- W2084283669 abstract "Understanding of protein structure and stability gained to date has been acquired through investigations made under dilute conditions where total macromolecular concentration never surpasses 10 g l(-1). However, biological macromolecules are known to evolve and function under crowded intracellular environments that comprises of proteins, nucleic acids, ribosomes and carbohydrates etc. Crowded environment is known to result in altered biological properties including thermodynamic, structural and functional aspect of macromolecules as compared to the macromolecules present in our commonly used experimental dilute buffers (for example, Tris HCl or phosphate buffer). In this study, we have investigated the thermodynamic and structural consequences of synthetic crowding agent (Ficoll 70) on three different proteins (Ribonuclease-A, lysozyme and holo α-lactalbumin) at different pH values. We report here that the effect of crowding is protein dependent in terms of protein thermal stability and structure. We also observed that the structural characteristics of the denatured state determines if crowding will have an effect or not on the protein stability." @default.
- W2084283669 created "2016-06-24" @default.
- W2084283669 creator A5036458901 @default.
- W2084283669 creator A5087877892 @default.
- W2084283669 date "2013-11-12" @default.
- W2084283669 modified "2023-09-27" @default.
- W2084283669 title "Denatured State Structural Property Determines Protein Stabilization by Macromolecular Crowding: A Thermodynamic and Structural Approach" @default.
- W2084283669 cites W1496368635 @default.
- W2084283669 cites W1565036272 @default.
- W2084283669 cites W1600015000 @default.
- W2084283669 cites W1974752106 @default.
- W2084283669 cites W1982946753 @default.
- W2084283669 cites W1987730349 @default.
- W2084283669 cites W1988517764 @default.
- W2084283669 cites W1989297730 @default.
- W2084283669 cites W1989920629 @default.
- W2084283669 cites W1992513829 @default.
- W2084283669 cites W1995838356 @default.
- W2084283669 cites W2003744642 @default.
- W2084283669 cites W2006512709 @default.
- W2084283669 cites W2015707255 @default.
- W2084283669 cites W2021592649 @default.
- W2084283669 cites W2029988455 @default.
- W2084283669 cites W2034728705 @default.
- W2084283669 cites W2036992699 @default.
- W2084283669 cites W2037658617 @default.
- W2084283669 cites W2041804296 @default.
- W2084283669 cites W2042767023 @default.
- W2084283669 cites W2045777307 @default.
- W2084283669 cites W2051273784 @default.
- W2084283669 cites W2054898507 @default.
- W2084283669 cites W2059139365 @default.
- W2084283669 cites W2064698049 @default.
- W2084283669 cites W2066736364 @default.
- W2084283669 cites W2069165179 @default.
- W2084283669 cites W2070018773 @default.
- W2084283669 cites W2078862798 @default.
- W2084283669 cites W2078933151 @default.
- W2084283669 cites W2083249145 @default.
- W2084283669 cites W2084572582 @default.
- W2084283669 cites W2085519055 @default.
- W2084283669 cites W2088858313 @default.
- W2084283669 cites W2105880172 @default.
- W2084283669 cites W2116076986 @default.
- W2084283669 cites W2131234140 @default.
- W2084283669 cites W2134972813 @default.
- W2084283669 cites W2136617021 @default.
- W2084283669 cites W2141687986 @default.
- W2084283669 cites W2147700328 @default.
- W2084283669 cites W2148746138 @default.
- W2084283669 cites W2151261370 @default.
- W2084283669 cites W2152163651 @default.
- W2084283669 cites W2155342128 @default.
- W2084283669 cites W2161486437 @default.
- W2084283669 cites W2165360511 @default.
- W2084283669 cites W2165847215 @default.
- W2084283669 cites W2168161204 @default.
- W2084283669 cites W2169113904 @default.
- W2084283669 cites W2315952176 @default.
- W2084283669 cites W2323551623 @default.
- W2084283669 cites W2325407743 @default.
- W2084283669 doi "https://doi.org/10.1371/journal.pone.0078936" @default.
- W2084283669 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3827121" @default.
- W2084283669 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24265729" @default.
- W2084283669 hasPublicationYear "2013" @default.
- W2084283669 type Work @default.
- W2084283669 sameAs 2084283669 @default.
- W2084283669 citedByCount "49" @default.
- W2084283669 countsByYear W20842836692014 @default.
- W2084283669 countsByYear W20842836692015 @default.
- W2084283669 countsByYear W20842836692016 @default.
- W2084283669 countsByYear W20842836692017 @default.
- W2084283669 countsByYear W20842836692018 @default.
- W2084283669 countsByYear W20842836692019 @default.
- W2084283669 countsByYear W20842836692020 @default.
- W2084283669 countsByYear W20842836692021 @default.
- W2084283669 countsByYear W20842836692022 @default.
- W2084283669 countsByYear W20842836692023 @default.
- W2084283669 crossrefType "journal-article" @default.
- W2084283669 hasAuthorship W2084283669A5036458901 @default.
- W2084283669 hasAuthorship W2084283669A5087877892 @default.
- W2084283669 hasBestOaLocation W20842836691 @default.
- W2084283669 hasConcept C104317684 @default.
- W2084283669 hasConcept C105168689 @default.
- W2084283669 hasConcept C12554922 @default.
- W2084283669 hasConcept C137061746 @default.
- W2084283669 hasConcept C181990884 @default.
- W2084283669 hasConcept C185592680 @default.
- W2084283669 hasConcept C191120209 @default.
- W2084283669 hasConcept C19472624 @default.
- W2084283669 hasConcept C202751555 @default.
- W2084283669 hasConcept C204328495 @default.
- W2084283669 hasConcept C204389451 @default.
- W2084283669 hasConcept C2776966407 @default.
- W2084283669 hasConcept C2778976237 @default.
- W2084283669 hasConcept C2909945666 @default.
- W2084283669 hasConcept C2988375501 @default.
- W2084283669 hasConcept C47701112 @default.