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- W2084296152 abstract "A new method for the isolation and separation of α-ketoacids participating in the citric acid cycle is described. The dinitrophenylhydrazones (DNPH) formed in the cold aqueous system are extracted with ether, removed from the ether with sodium carbonate, re-extracted with ether after acidification, and subjected to descending chromatography on a paper impregnated with phosphate buffer. The solvent consists of n-butanol pre-treated with an equal volume 3% aqueous ammonia. Pyruvic DNPH gives two spots (RF = 0.61 and 0.75), oxalacetic and α-ketoglutaric DNPH each one spot only (RF = 0.10 and 0.07, respectively). After elution, the hydrazones are determined by colorimetry. Two micrograms of the acids can still be determined, and a check on recovery is possible by isotope dilution. Oxalacetic DNPH is stable at room temperature, even when dissolved in 10% Na2CO3, but is decomposed to pyruvic DNPH by moderate heat. Therefore, in previous investigations where oxalacetic DNPH had been exposed to heat during analysis, major errors must have occurred." @default.
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- W2084296152 date "1959-01-01" @default.
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- W2084296152 title "Chromatographic separation of pyruvic, oxalacetic and alpha-ketoglutaric acid from tissue cultures" @default.
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- W2084296152 doi "https://doi.org/10.1016/s0021-9673(01)86266-x" @default.
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