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- W2084348570 abstract "Liquid water is essential to life on earth. The cell, the basic component of all living organisms, functions through the action of proteins in a liquid environment. Consequently, the ability to directly visualize protein structure at nanometer-resolution in a liquid environment would dramatically advance mechanistic understanding of protein function in cellular activity. Current techniques in imaging biological samples in liquid solution are limited by micrometer scale resolution, Fluorescence-labeled, dehydrated, fixed, stained or frozen in ice.Here, we report an electron microscopic approach for imaging label-free, near-native biological samples in liquid environment. By passing electrons through a thin layer of liquid-buffer sealed within a micro-chamber, we directly visualized the structure of proteins, viruses, bacteria and human cells at nanometer resolution, even achieved their 3D structures. For example, the electron tomographic 3D reconstruction (Figure) of a label-free living bacteria, Magnetospirillum magneticum in growth medium showed a smooth outer shell and internal components, such as poly(3-hydroxybutyrate) (PHB), chromosomal mass (CR), magnetosomes (MG) and internal septal layer. These results suggest the method offers both a general-purpose and high-throughput tool for imaging the structure of biological samples in near-native physiological liquid conditions.View Large Image | View Hi-Res Image | Download PowerPoint Slide" @default.
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- W2084348570 date "2014-01-01" @default.
- W2084348570 modified "2023-09-28" @default.
- W2084348570 title "Visualizing Biological Samples in Liquid Solution by Electron Microscopy" @default.
- W2084348570 doi "https://doi.org/10.1016/j.bpj.2013.11.3311" @default.
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