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- W2084360148 abstract "The volumetric changes, structural behavior, and subsequent survival rate of juvenileNereis (Neanthes) virens(Annelida: Polychaeta) at the three chaetiger stage of development have been observed during equilibrium and nonequilibrium low-temperature dehydration in a controlled-rate freezer and on the stage of a planar cryomicroscope. Using a cooling rate 0.3°C/min and 1.4MMe2SO, substantial levels of larval survival were observed following transfer to liquid N from a temperature of −25°C when the equilibrium fractional water volume is expected to be 7.1%. The cryopreservation under equilibrium conditions is thought to indicate vitrification of the internal Me2SO which will have reached 54% at −25°C. When a cooling rate of 2.5°C/min was used, the long-term survival rate was substantially greater, reaching 86%, depending on developmental stage following transfer to liquid N from a temperature of −35°C." @default.
- W2084360148 created "2016-06-24" @default.
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- W2084360148 date "1997-05-01" @default.
- W2084360148 modified "2023-09-25" @default.
- W2084360148 title "Cryopreservation ofNereis virens(Polychaeta, Annelida) Larvae: The Mechanism of Cryopreservation of a Differentiated Metazoan" @default.
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- W2084360148 doi "https://doi.org/10.1006/cryo.1997.2006" @default.
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