FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2084416013> ?p ?o ?g. }

• W2084416013 endingPage "23" @default.
• W2084416013 startingPage "15" @default.
• W2084416013 abstract "Porcine reproductive and respiratory syndrome virus (PRRSV) is an Arterivirus recognised world wide as an important cause of reproductive failure and pneumonia in pigs. American and European strains of PRRSV, differentiated antigenically and genomically, have been reported. PRRSV infections are currently diagnosed using serology, virus isolation and/or immunocytochemistry. In order to overcome various drawbacks associated with these techniques, conventional, block-based RT-PCR methods for the detection of PRRSV nucleic acid in clinical samples have been described. These methods require gel electrophoresis for analysis of PCR products and present high risk of DNA carry-over contamination between the samples tested. We describe the detection of PRRSV RNA in serum samples and in blood impregnated filter disks (FDs), obtained from experimentally inoculated pigs, using a closed-tube, fluorimeter-based PCR assay. The assay eliminates the use of gel electrophoresis, and is as sensitive and specific as the conventional block-based PCR assay, detecting positive samples as early as 1 day post-inoculation. We also report a rapid fluorimeter based PCR method for differentiating American and European strains of PRRSV." @default.
• W2084416013 created "2016-06-24" @default.
• W2084416013 creator A5013601236 @default.
• W2084416013 creator A5033723594 @default.
• W2084416013 creator A5038568975 @default.
• W2084416013 creator A5063555821 @default.
• W2084416013 creator A5073080424 @default.
• W2084416013 creator A5079215140 @default.
• W2084416013 date "2000-09-01" @default.
• W2084416013 modified "2023-10-10" @default.
• W2084416013 title "Rapid detection of porcine reproductive and respiratory syndrome viral nucleic acid in blood using a fluorimeter based PCR method" @default.
• W2084416013 cites W1569947224 @default.
• W2084416013 cites W1679554208 @default.
• W2084416013 cites W1812491466 @default.
• W2084416013 cites W1828422676 @default.
• W2084416013 cites W1964227218 @default.
• W2084416013 cites W1979633272 @default.
• W2084416013 cites W2011698864 @default.
• W2084416013 cites W2022506220 @default.
• W2084416013 cites W2026125064 @default.
• W2084416013 cites W2031457110 @default.
• W2084416013 cites W2043286293 @default.
• W2084416013 cites W2044725705 @default.
• W2084416013 cites W2046274883 @default.
• W2084416013 cites W2070518148 @default.
• W2084416013 cites W2089186775 @default.
• W2084416013 cites W2094872956 @default.
• W2084416013 cites W2096360159 @default.
• W2084416013 cites W2121856068 @default.
• W2084416013 cites W2129620275 @default.
• W2084416013 cites W2142950123 @default.
• W2084416013 cites W2150705960 @default.
• W2084416013 cites W2162945607 @default.
• W2084416013 cites W4254533614 @default.
• W2084416013 doi "https://doi.org/10.1016/s0378-1135(00)00222-4" @default.
• W2084416013 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10925037" @default.
• W2084416013 hasPublicationYear "2000" @default.
• W2084416013 type Work @default.
• W2084416013 sameAs 2084416013 @default.
• W2084416013 citedByCount "13" @default.
• W2084416013 countsByYear W20844160132015 @default.
• W2084416013 countsByYear W20844160132018 @default.
• W2084416013 countsByYear W20844160132020 @default.
• W2084416013 crossrefType "journal-article" @default.
• W2084416013 hasAuthorship W2084416013A5013601236 @default.
• W2084416013 hasAuthorship W2084416013A5033723594 @default.
• W2084416013 hasAuthorship W2084416013A5038568975 @default.
• W2084416013 hasAuthorship W2084416013A5063555821 @default.
• W2084416013 hasAuthorship W2084416013A5073080424 @default.
• W2084416013 hasAuthorship W2084416013A5079215140 @default.
• W2084416013 hasConcept C104317684 @default.
• W2084416013 hasConcept C116084860 @default.
• W2084416013 hasConcept C121332964 @default.
• W2084416013 hasConcept C142724271 @default.
• W2084416013 hasConcept C153911025 @default.
• W2084416013 hasConcept C159047783 @default.
• W2084416013 hasConcept C202800777 @default.
• W2084416013 hasConcept C24107716 @default.
• W2084416013 hasConcept C2522874641 @default.
• W2084416013 hasConcept C2779134260 @default.
• W2084416013 hasConcept C2779656172 @default.
• W2084416013 hasConcept C2779819720 @default.
• W2084416013 hasConcept C3008058167 @default.
• W2084416013 hasConcept C48023723 @default.
• W2084416013 hasConcept C49105822 @default.
• W2084416013 hasConcept C524204448 @default.
• W2084416013 hasConcept C54355233 @default.
• W2084416013 hasConcept C62520636 @default.
• W2084416013 hasConcept C71924100 @default.
• W2084416013 hasConcept C86803240 @default.
• W2084416013 hasConcept C91881484 @default.
• W2084416013 hasConceptScore W2084416013C104317684 @default.
• W2084416013 hasConceptScore W2084416013C116084860 @default.
• W2084416013 hasConceptScore W2084416013C121332964 @default.
• W2084416013 hasConceptScore W2084416013C142724271 @default.
• W2084416013 hasConceptScore W2084416013C153911025 @default.
• W2084416013 hasConceptScore W2084416013C159047783 @default.
• W2084416013 hasConceptScore W2084416013C202800777 @default.
• W2084416013 hasConceptScore W2084416013C24107716 @default.
• W2084416013 hasConceptScore W2084416013C2522874641 @default.
• W2084416013 hasConceptScore W2084416013C2779134260 @default.
• W2084416013 hasConceptScore W2084416013C2779656172 @default.
• W2084416013 hasConceptScore W2084416013C2779819720 @default.
• W2084416013 hasConceptScore W2084416013C3008058167 @default.
• W2084416013 hasConceptScore W2084416013C48023723 @default.
• W2084416013 hasConceptScore W2084416013C49105822 @default.
• W2084416013 hasConceptScore W2084416013C524204448 @default.
• W2084416013 hasConceptScore W2084416013C54355233 @default.
• W2084416013 hasConceptScore W2084416013C62520636 @default.
• W2084416013 hasConceptScore W2084416013C71924100 @default.
• W2084416013 hasConceptScore W2084416013C86803240 @default.
• W2084416013 hasConceptScore W2084416013C91881484 @default.
• W2084416013 hasIssue "1" @default.
• W2084416013 hasLocation W20844160131 @default.
• W2084416013 hasLocation W20844160132 @default.
• W2084416013 hasOpenAccess W2084416013 @default.
• W2084416013 hasPrimaryLocation W20844160131 @default.
• W2084416013 hasRelatedWork W109151143 @default.

• next