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- W2084431791 abstract "High-molecular-weight DNA was extracted from livers of Glis glis (L.) to create a small- insert genomic library constructed in the M13mp18 phage vector. The primary library consisted of 30,000 clones. Primer extension enrichment and a biotin/streptavidin capture technique were applied to obtain enriched sublibraries containing inserts with (CA)n and (GA)n motifs. One-third of the resulting clones that were screened for CA-repeats contained non-redundant sequences with more than 6 repeats, and sufficient size of insert-flanking region to design PCR primers. Less than 10% of clones that were screened for (GA) n microsatellites contained repetitive sequences, and none of them was useful for primer design. The average clone size of the positive clones was ca 350 bp." @default.
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- W2084431791 date "2003-01-01" @default.
- W2084431791 modified "2023-09-27" @default.
- W2084431791 title "Small-insert genomic DNA library of Glis glis (L.) useful for microsatellite isolation" @default.
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