FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2084523310> ?p ?o ?g. }

• W2084523310 endingPage "1873" @default.
• W2084523310 startingPage "1861" @default.
• W2084523310 abstract "In mice whose Gi/o-protein function had been impaired by antisense 'knock-down' or pertussis toxin treatment, i.c.v. injection of myr+-Gi/o alpha subunits restored the effectiveness of beta-endorphin, morphine, DPDPE, clonidine and neurotensin to produce antinociception. Myr+-G alpha subunits of the class of G-proteins actually impaired were more effective than unlike but related myr+-G alpha subunits. Selectivity was noted in that only exogenous myr+-G alpha subunits affected (enhanced) the activity of agonists in G alpha-deficient signalling systems. This treatment had little effect on agonist potency when the impairment resided at the receptor level. The potential of the opioids, clonidine and R-PIA to increase G alpha-related in vitro hydrolysis of GTP was also re-established after injecting myr+-Gi2 alpha subunits into Gi2-knocked-down mice. Myr+-Gi2 alpha subunits pre-incubated with GTPgammaS or GDPbetaS before i.c.v. injection did not improve the activity of agonists in vivo (antinociception) or in vitro (regulation of low Km GTPase). After impairing the function of PKCbeta1 by antisense treatment or with the inhibitor H7, the effect of myr+-G alpha subunits on agonist potency was prevented. Electron microscope analysis showed the entry of gold-conjugated myr+-G alpha subunits into neural cells. These particles were found in the cytoplasm, associated with the plasma membranes of different neuronal processes and also in synaptic junctions. In cultured neurons and astrocytes myr+-Gi2 alpha-associated fluorescence was internalised in a dose-dependent manner and distributed in the plasma membrane and cytosol, as well as in nuclei of dividing astrocytes. Thus, G alpha subunits in CSF enter into neurons and functionally couple to the receptor-triggered signalling cascade. As G-proteins have been implicated in the pathophysiology of several neural disorders, this finding may be valuable in the therapy of such dysfunctions." @default.
• W2084523310 created "2016-06-24" @default.
• W2084523310 creator A5000293437 @default.
• W2084523310 creator A5019701584 @default.
• W2084523310 creator A5040552736 @default.
• W2084523310 creator A5051380834 @default.
• W2084523310 creator A5062399928 @default.
• W2084523310 creator A5090552628 @default.
• W2084523310 date "1999-12-01" @default.
• W2084523310 modified "2023-10-18" @default.
• W2084523310 title "Myr+-Gi2α and Goα subunits restore the efficacy of opioids, clonidine and neurotensin giving rise to antinociception in G-protein knock-down mice" @default.
• W2084523310 cites W1493004256 @default.
• W2084523310 cites W1496619420 @default.
• W2084523310 cites W1499240512 @default.
• W2084523310 cites W1522062003 @default.
• W2084523310 cites W1567645629 @default.
• W2084523310 cites W1585650753 @default.
• W2084523310 cites W1921104055 @default.
• W2084523310 cites W1974497078 @default.
• W2084523310 cites W1975436627 @default.
• W2084523310 cites W1975651701 @default.
• W2084523310 cites W1977938288 @default.
• W2084523310 cites W1979067492 @default.
• W2084523310 cites W1984787336 @default.
• W2084523310 cites W2001668713 @default.
• W2084523310 cites W2008286131 @default.
• W2084523310 cites W2036229899 @default.
• W2084523310 cites W2040882563 @default.
• W2084523310 cites W2059588956 @default.
• W2084523310 cites W2059994807 @default.
• W2084523310 cites W2076798747 @default.
• W2084523310 cites W2080533763 @default.
• W2084523310 cites W2081128554 @default.
• W2084523310 cites W2086140963 @default.
• W2084523310 cites W2089581769 @default.
• W2084523310 cites W2091006667 @default.
• W2084523310 cites W2093407362 @default.
• W2084523310 cites W2093514167 @default.
• W2084523310 cites W2093750177 @default.
• W2084523310 cites W2094823280 @default.
• W2084523310 cites W2110408216 @default.
• W2084523310 cites W2131512973 @default.
• W2084523310 cites W2132510575 @default.
• W2084523310 cites W2140686653 @default.
• W2084523310 cites W2316337921 @default.
• W2084523310 cites W2346553109 @default.
• W2084523310 cites W269476549 @default.
• W2084523310 cites W282055414 @default.
• W2084523310 doi "https://doi.org/10.1016/s0028-3908(99)00070-2" @default.
• W2084523310 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10608281" @default.
• W2084523310 hasPublicationYear "1999" @default.
• W2084523310 type Work @default.
• W2084523310 sameAs 2084523310 @default.
• W2084523310 citedByCount "16" @default.
• W2084523310 countsByYear W20845233102013 @default.
• W2084523310 crossrefType "journal-article" @default.
• W2084523310 hasAuthorship W2084523310A5000293437 @default.
• W2084523310 hasAuthorship W2084523310A5019701584 @default.
• W2084523310 hasAuthorship W2084523310A5040552736 @default.
• W2084523310 hasAuthorship W2084523310A5051380834 @default.
• W2084523310 hasAuthorship W2084523310A5062399928 @default.
• W2084523310 hasAuthorship W2084523310A5090552628 @default.
• W2084523310 hasConcept C104317684 @default.
• W2084523310 hasConcept C118303440 @default.
• W2084523310 hasConcept C126322002 @default.
• W2084523310 hasConcept C134018914 @default.
• W2084523310 hasConcept C141231307 @default.
• W2084523310 hasConcept C159110408 @default.
• W2084523310 hasConcept C170493617 @default.
• W2084523310 hasConcept C185592680 @default.
• W2084523310 hasConcept C24389824 @default.
• W2084523310 hasConcept C2775944032 @default.
• W2084523310 hasConcept C2778399450 @default.
• W2084523310 hasConcept C2778684643 @default.
• W2084523310 hasConcept C2778938600 @default.
• W2084523310 hasConcept C2778976297 @default.
• W2084523310 hasConcept C49453240 @default.
• W2084523310 hasConcept C55493867 @default.
• W2084523310 hasConcept C64943373 @default.
• W2084523310 hasConcept C71924100 @default.
• W2084523310 hasConcept C80631254 @default.
• W2084523310 hasConcept C86803240 @default.
• W2084523310 hasConcept C98274493 @default.
• W2084523310 hasConceptScore W2084523310C104317684 @default.
• W2084523310 hasConceptScore W2084523310C118303440 @default.
• W2084523310 hasConceptScore W2084523310C126322002 @default.
• W2084523310 hasConceptScore W2084523310C134018914 @default.
• W2084523310 hasConceptScore W2084523310C141231307 @default.
• W2084523310 hasConceptScore W2084523310C159110408 @default.
• W2084523310 hasConceptScore W2084523310C170493617 @default.
• W2084523310 hasConceptScore W2084523310C185592680 @default.
• W2084523310 hasConceptScore W2084523310C24389824 @default.
• W2084523310 hasConceptScore W2084523310C2775944032 @default.
• W2084523310 hasConceptScore W2084523310C2778399450 @default.
• W2084523310 hasConceptScore W2084523310C2778684643 @default.
• W2084523310 hasConceptScore W2084523310C2778938600 @default.
• W2084523310 hasConceptScore W2084523310C2778976297 @default.
• W2084523310 hasConceptScore W2084523310C49453240 @default.

• next