Matches in SemOpenAlex for { <https://semopenalex.org/work/W2084545829> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W2084545829 endingPage "54" @default.
- W2084545829 startingPage "51" @default.
- W2084545829 abstract "In this paper, the potential role of aphids in viroid transmission was explored. Apterous aphids were fed on pospiviroid-infected plants and viroid targets in the aphids were consequently quantified through RT-qPCR and localized within the aphid body using fluorescence in situ hybridization (FISH). Based on the analytical sensitivity test, the limit of detection (LOD) was estimated at 1.69 × 106 viroid copies per individual aphid body. To localize the viroids in the aphids, a pospiviroid-generic Cy5-labelled probe was used and the fluorescent signal was determined by confocal microscopy. Viroids were clearly observed in the aphid's stylet and stomach, but not in the embryos. Viroids were detected in 29% of the aphids after a 24 h feeding period, which suggests only a partial and low concentration viroid uptake by the aphid population including viroid concentrations under the LOD. However, these results show that viroids can be ingested by aphids while feeding on infected plants, thus potentially increasing the transmission risk. The combination of FISH and RT-qPCR provides reliable and fast localization and quantitation of viroid targets in individual aphids and thus constitutes a valuable tool in future epidemiological research." @default.
- W2084545829 created "2016-06-24" @default.
- W2084545829 creator A5020993605 @default.
- W2084545829 creator A5039765697 @default.
- W2084545829 creator A5048770137 @default.
- W2084545829 creator A5050259630 @default.
- W2084545829 creator A5066515722 @default.
- W2084545829 date "2015-01-01" @default.
- W2084545829 modified "2023-10-16" @default.
- W2084545829 title "Quantitation and localization of pospiviroids in aphids" @default.
- W2084545829 cites W1493408495 @default.
- W2084545829 cites W1593960337 @default.
- W2084545829 cites W1984017239 @default.
- W2084545829 cites W1984670251 @default.
- W2084545829 cites W1993990319 @default.
- W2084545829 cites W2012879944 @default.
- W2084545829 cites W2032946853 @default.
- W2084545829 cites W2034088647 @default.
- W2084545829 cites W2045392465 @default.
- W2084545829 cites W2052967966 @default.
- W2084545829 cites W2069701565 @default.
- W2084545829 cites W2071866475 @default.
- W2084545829 cites W2075159829 @default.
- W2084545829 cites W2096249174 @default.
- W2084545829 cites W2125025413 @default.
- W2084545829 cites W2148773326 @default.
- W2084545829 cites W2149340979 @default.
- W2084545829 cites W2169796828 @default.
- W2084545829 cites W80054073 @default.
- W2084545829 doi "https://doi.org/10.1016/j.jviromet.2014.10.003" @default.
- W2084545829 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25455904" @default.
- W2084545829 hasPublicationYear "2015" @default.
- W2084545829 type Work @default.
- W2084545829 sameAs 2084545829 @default.
- W2084545829 citedByCount "16" @default.
- W2084545829 countsByYear W20845458292015 @default.
- W2084545829 countsByYear W20845458292016 @default.
- W2084545829 countsByYear W20845458292017 @default.
- W2084545829 countsByYear W20845458292018 @default.
- W2084545829 countsByYear W20845458292019 @default.
- W2084545829 countsByYear W20845458292020 @default.
- W2084545829 countsByYear W20845458292021 @default.
- W2084545829 countsByYear W20845458292023 @default.
- W2084545829 crossrefType "journal-article" @default.
- W2084545829 hasAuthorship W2084545829A5020993605 @default.
- W2084545829 hasAuthorship W2084545829A5039765697 @default.
- W2084545829 hasAuthorship W2084545829A5048770137 @default.
- W2084545829 hasAuthorship W2084545829A5050259630 @default.
- W2084545829 hasAuthorship W2084545829A5066515722 @default.
- W2084545829 hasConcept C109110057 @default.
- W2084545829 hasConcept C144024400 @default.
- W2084545829 hasConcept C149923435 @default.
- W2084545829 hasConcept C159047783 @default.
- W2084545829 hasConcept C2522874641 @default.
- W2084545829 hasConcept C2775976403 @default.
- W2084545829 hasConcept C2777066214 @default.
- W2084545829 hasConcept C2780757758 @default.
- W2084545829 hasConcept C2780781446 @default.
- W2084545829 hasConcept C2908647359 @default.
- W2084545829 hasConcept C59822182 @default.
- W2084545829 hasConcept C86803240 @default.
- W2084545829 hasConceptScore W2084545829C109110057 @default.
- W2084545829 hasConceptScore W2084545829C144024400 @default.
- W2084545829 hasConceptScore W2084545829C149923435 @default.
- W2084545829 hasConceptScore W2084545829C159047783 @default.
- W2084545829 hasConceptScore W2084545829C2522874641 @default.
- W2084545829 hasConceptScore W2084545829C2775976403 @default.
- W2084545829 hasConceptScore W2084545829C2777066214 @default.
- W2084545829 hasConceptScore W2084545829C2780757758 @default.
- W2084545829 hasConceptScore W2084545829C2780781446 @default.
- W2084545829 hasConceptScore W2084545829C2908647359 @default.
- W2084545829 hasConceptScore W2084545829C59822182 @default.
- W2084545829 hasConceptScore W2084545829C86803240 @default.
- W2084545829 hasLocation W20845458291 @default.
- W2084545829 hasLocation W20845458292 @default.
- W2084545829 hasOpenAccess W2084545829 @default.
- W2084545829 hasPrimaryLocation W20845458291 @default.
- W2084545829 hasRelatedWork W1528149948 @default.
- W2084545829 hasRelatedWork W2010179970 @default.
- W2084545829 hasRelatedWork W2039495325 @default.
- W2084545829 hasRelatedWork W2084545829 @default.
- W2084545829 hasRelatedWork W2103389055 @default.
- W2084545829 hasRelatedWork W2589541615 @default.
- W2084545829 hasRelatedWork W2905702912 @default.
- W2084545829 hasRelatedWork W4316015916 @default.
- W2084545829 hasRelatedWork W4382344662 @default.
- W2084545829 hasRelatedWork W80054073 @default.
- W2084545829 hasVolume "211" @default.
- W2084545829 isParatext "false" @default.
- W2084545829 isRetracted "false" @default.
- W2084545829 magId "2084545829" @default.
- W2084545829 workType "article" @default.