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- W2084607718 abstract "The accurate measurement of soil nitrate (NO3−) is critical for determining rates of nitrogen (N) cycling and potential N losses from ecosystems. Iron (Fe) can interfere with the colorimetric NO3− analysis of soil extracts to cause the appearance of anomalously low NO3− concentrations. To resolve the interference, imidazole or NH4Cl–DTPA has been recommended to replace NH4Cl–EDTA as the buffer in the analysis. Here we show that phosphate (PO43−) filtration can completely remove Fe interference whereas any of these buffers alone may not. Regardless of which buffer was used, 5.5–55 mg Fe L−1 ferrous iron (Fe2+) interfered with NO3− determination in 0.3 mg N–NO3− L−1 2 M KCl solutions. Phosphate filtration removed all detectable Fe2+ in 2 M KCl anaerobic soil slurry extracts with high Fe2+ concentrations (25.9 ± 1.7 mg Fe2+ L−1). With each of the three buffers tested, the measured NO3− concentrations in the anaerobic soil slurry extracts were significantly higher with PO43− filtration compared to without filtration. After filtration, the measured NO3− concentrations were similar across all three buffers, suggesting that NO3− concentrations were accurately measured in PO43− filtered soil extracts regardless of the buffer used. The Fe:N ratio of Fe interference with NO3− determination depended on Fe concentration, NO3− concentration, buffer, and cadmium column age, so that the amount of Fe interference that could occur can be difficult to predict. We suggest comparing measured NO3− concentrations for unfiltered and PO43− filtered soil extracts to determine the potential for Fe interference in colorimetric NO3− determination as standard additions may not detect all forms of Fe interference." @default.
- W2084607718 created "2016-06-24" @default.
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- W2084607718 date "2012-03-01" @default.
- W2084607718 modified "2023-09-25" @default.
- W2084607718 title "A new approach for removing iron interference from soil nitrate analysis" @default.
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- W2084607718 doi "https://doi.org/10.1016/j.soilbio.2011.12.003" @default.
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