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- W2084667040 abstract "Identification of the subcellular localization of a particular protein provides essential information towards understanding its physiological function. Among other techniques, aqueous polymer two-phase partitioning (APTP) has become a widely used method to purify plant plasma membranes (PMs), and, consequently, to support claims for the association of a protein of interest with that membrane type. For example, in a recent paper [1], the involvement of a PM NADPH oxidase in abscisic acid- and water stress-induced antioxidant defense responses was suggested mainly on the basis of APTP. As is common practice, APTP-purified PM vesicles were prepared under iso-osmotic and low ionic strength conditions, and activity assays were performed using hypo-osmotic and low ionic strength buffers. However, it has been repeatedly shown that proteins can non-specifically associate to membranes under these conditions and therefore their unequivocal localization to the PM requires more thoughtful approaches. Here, we provide a critical analysis of membrane isolation conditions and suggestions to prevent premature conclusions." @default.
- W2084667040 created "2016-06-24" @default.
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- W2084667040 date "2003-06-01" @default.
- W2084667040 modified "2023-10-18" @default.
- W2084667040 title "Soluble proteins, an often overlooked contaminant in plasma membrane preparations" @default.
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- W2084667040 doi "https://doi.org/10.1016/s1360-1385(03)00100-6" @default.
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