Matches in SemOpenAlex for { <https://semopenalex.org/work/W2084772983> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2084772983 endingPage "174" @default.
- W2084772983 startingPage "171" @default.
- W2084772983 abstract "Rab proteins are ras‐like low molecular mass GTP‐binding proteins, which are postulated to act as specific regulators of membrane trafficking in exocytosis and endocytosis. We have previously shown that synthetic peptides, corresponding to the effector domain of Rab3 proteins, stimulate a complete exocytotic response in mast cells. We have used a PCR‐eloning strategy to investigate the presence of mRNA encoding Rab3 in mast cells. RNA based PCR was then performed on mast cell RNA using degenerate oligonucleotide primers based on two conserved sequences among Rab3 proteins. However, no PCR products were obtained, even for proteins known to be expressed in high copy numbers in mast cells (β‐actin and Fc receptor). We have found that the problem resides in the presence of mast cell secretory granule derived heparin, that copurifies with the RNA; heparin has been shown to inhibit the activity of reverse transcriptase and Taq polymerase in PCR. After treating the RNA (obtained from about 500 mast cells) with heparinase, several PCR products of varying size were obtained using primers specific for Rab3 proteins. These products were cloned and sequenced. We have found clones containing sequences that had a 100% homology at the deduced amino acid level to a portion of Rab3B and Rab3D (amino acids 16 to 83)." @default.
- W2084772983 created "2016-06-24" @default.
- W2084772983 creator A5035393323 @default.
- W2084772983 creator A5042922819 @default.
- W2084772983 creator A5049272540 @default.
- W2084772983 creator A5072944023 @default.
- W2084772983 date "1994-02-14" @default.
- W2084772983 modified "2023-10-11" @default.
- W2084772983 title "RT‐PCR cloning of Rab3 isoforms expressed in peritoneal mast cells" @default.
- W2084772983 cites W1498711077 @default.
- W2084772983 cites W1528764883 @default.
- W2084772983 cites W1539163638 @default.
- W2084772983 cites W1553798208 @default.
- W2084772983 cites W1832730936 @default.
- W2084772983 cites W195281982 @default.
- W2084772983 cites W1966376079 @default.
- W2084772983 cites W1977598234 @default.
- W2084772983 cites W1985697692 @default.
- W2084772983 cites W1988420272 @default.
- W2084772983 cites W1996462307 @default.
- W2084772983 cites W1998783767 @default.
- W2084772983 cites W2003862885 @default.
- W2084772983 cites W2004212715 @default.
- W2084772983 cites W2004788546 @default.
- W2084772983 cites W2005489113 @default.
- W2084772983 cites W2007266423 @default.
- W2084772983 cites W2011737878 @default.
- W2084772983 cites W2014197706 @default.
- W2084772983 cites W2017629118 @default.
- W2084772983 cites W2025972769 @default.
- W2084772983 cites W2026841478 @default.
- W2084772983 cites W2036958086 @default.
- W2084772983 cites W2039334467 @default.
- W2084772983 cites W2052988804 @default.
- W2084772983 cites W2076730826 @default.
- W2084772983 cites W2076873399 @default.
- W2084772983 cites W2079683808 @default.
- W2084772983 cites W2116743411 @default.
- W2084772983 cites W2130398497 @default.
- W2084772983 cites W2140962689 @default.
- W2084772983 cites W2145065899 @default.
- W2084772983 cites W2339022261 @default.
- W2084772983 cites W4294216491 @default.
- W2084772983 doi "https://doi.org/10.1016/0014-5793(94)80409-5" @default.
- W2084772983 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7508866" @default.
- W2084772983 hasPublicationYear "1994" @default.
- W2084772983 type Work @default.
- W2084772983 sameAs 2084772983 @default.
- W2084772983 citedByCount "41" @default.
- W2084772983 countsByYear W20847729832013 @default.
- W2084772983 crossrefType "journal-article" @default.
- W2084772983 hasAuthorship W2084772983A5035393323 @default.
- W2084772983 hasAuthorship W2084772983A5042922819 @default.
- W2084772983 hasAuthorship W2084772983A5049272540 @default.
- W2084772983 hasAuthorship W2084772983A5072944023 @default.
- W2084772983 hasConcept C104317684 @default.
- W2084772983 hasConcept C105580179 @default.
- W2084772983 hasConcept C153911025 @default.
- W2084772983 hasConcept C156728348 @default.
- W2084772983 hasConcept C203014093 @default.
- W2084772983 hasConcept C207332259 @default.
- W2084772983 hasConcept C2779726688 @default.
- W2084772983 hasConcept C55493867 @default.
- W2084772983 hasConcept C67705224 @default.
- W2084772983 hasConcept C86803240 @default.
- W2084772983 hasConcept C95444343 @default.
- W2084772983 hasConceptScore W2084772983C104317684 @default.
- W2084772983 hasConceptScore W2084772983C105580179 @default.
- W2084772983 hasConceptScore W2084772983C153911025 @default.
- W2084772983 hasConceptScore W2084772983C156728348 @default.
- W2084772983 hasConceptScore W2084772983C203014093 @default.
- W2084772983 hasConceptScore W2084772983C207332259 @default.
- W2084772983 hasConceptScore W2084772983C2779726688 @default.
- W2084772983 hasConceptScore W2084772983C55493867 @default.
- W2084772983 hasConceptScore W2084772983C67705224 @default.
- W2084772983 hasConceptScore W2084772983C86803240 @default.
- W2084772983 hasConceptScore W2084772983C95444343 @default.
- W2084772983 hasIssue "1-2" @default.
- W2084772983 hasLocation W20847729831 @default.
- W2084772983 hasLocation W20847729832 @default.
- W2084772983 hasOpenAccess W2084772983 @default.
- W2084772983 hasPrimaryLocation W20847729831 @default.
- W2084772983 hasRelatedWork W1566080932 @default.
- W2084772983 hasRelatedWork W197612135 @default.
- W2084772983 hasRelatedWork W1989587437 @default.
- W2084772983 hasRelatedWork W1992470677 @default.
- W2084772983 hasRelatedWork W2064186407 @default.
- W2084772983 hasRelatedWork W2082387821 @default.
- W2084772983 hasRelatedWork W2086823495 @default.
- W2084772983 hasRelatedWork W2116252656 @default.
- W2084772983 hasRelatedWork W2765379172 @default.
- W2084772983 hasRelatedWork W847395602 @default.
- W2084772983 hasVolume "339" @default.
- W2084772983 isParatext "false" @default.
- W2084772983 isRetracted "false" @default.
- W2084772983 magId "2084772983" @default.
- W2084772983 workType "article" @default.