FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2084790690> ?p ?o ?g. }

• W2084790690 abstract "In the vasculature, misdirected apoptosis in endothelial cells leads to pathological conditions such as inflammation. Along with biochemical and molecular signals, the hemodynamic forces that the cells experience are also important regulators of endothelial functions such as proliferation and apoptosis. Laminar shear stress inhibits apoptosis induced by serum depletion, oxidative stress, and tumor necrosis factor α (TNFα). Death associated protein kinase (DAPK) is a positive regulator of TNFα induced apoptotic pathway. Here we investigate the effect of shear stress on DAPK in endothelial cells on glass or silicone membrane substrate. We have already shown a link between shear stress and DAPK expression and apoptosis in cells on glass. Here we transition our study to endothelial cells on non-glass substrates, such as flexible silicone membrane used for cyclic strain studies. We modified the classic parallel plate flow chamber to accommodate silicone membrane as substrate for cells, and validated the chamber for cell viability in shear stress experiments. We found that adding shear stress significantly suppressed TNFα induced apoptosis in cells; while shearing cells alone also increased apoptosis on either substrate. We also found that shearing cells at 12 dynes/cm2 for 6 hours resulted in increased apoptosis on both substrates. This shear-induced apoptosis correlated with increased caspase 3/7 activities and DAPK expression and activation via dephosphorylation of serine 308. These data suggest that shear stress induced apoptosis in endothelial cells via increased DAPK expression and activation as well as caspase-3/7 activity. Most in vitro shear stress studies utilize the conventional parallel plate flow chamber where cells are cultured on glass, which is much stiffer than what cells encounter in vivo. Other mechanotransduction studies have utilized the flexible silicone membrane as substrate, for example, in cyclic stretch studies. Thus, this study bridges the gap between shear stress studies on cells plated on glass to studies on different stiffness of substrates or mechanical stimulation such as cyclic strain. We continue to explore the mechanotransduction role of DAPK in endothelial apoptosis, by using substrates of physiological stiffness for shear stress studies, and by using silicone substrate in cyclic stretch devices." @default.
• W2084790690 created "2016-06-24" @default.
• W2084790690 creator A5050143057 @default.
• W2084790690 creator A5076485897 @default.
• W2084790690 date "2013-01-10" @default.
• W2084790690 modified "2023-10-16" @default.
• W2084790690 title "Effect of shear stress and substrate on endothelial DAPK expression, caspase activity, and apoptosis" @default.
• W2084790690 cites W1717397784 @default.
• W2084790690 cites W1936770188 @default.
• W2084790690 cites W1966134982 @default.
• W2084790690 cites W1967091709 @default.
• W2084790690 cites W1968903390 @default.
• W2084790690 cites W1990126127 @default.
• W2084790690 cites W1992544868 @default.
• W2084790690 cites W1993269272 @default.
• W2084790690 cites W2014171174 @default.
• W2084790690 cites W2018313026 @default.
• W2084790690 cites W2021435809 @default.
• W2084790690 cites W2021646389 @default.
• W2084790690 cites W2023019714 @default.
• W2084790690 cites W2024250498 @default.
• W2084790690 cites W2025430706 @default.
• W2084790690 cites W2046986640 @default.
• W2084790690 cites W2052373675 @default.
• W2084790690 cites W2054635074 @default.
• W2084790690 cites W2057190622 @default.
• W2084790690 cites W2063563698 @default.
• W2084790690 cites W2065577350 @default.
• W2084790690 cites W2065591461 @default.
• W2084790690 cites W2074300163 @default.
• W2084790690 cites W2084788245 @default.
• W2084790690 cites W2092828187 @default.
• W2084790690 cites W2095301611 @default.
• W2084790690 cites W2118440238 @default.
• W2084790690 cites W2119892103 @default.
• W2084790690 cites W2127488841 @default.
• W2084790690 cites W2138752384 @default.
• W2084790690 cites W2139062484 @default.
• W2084790690 cites W2148328620 @default.
• W2084790690 cites W2161466396 @default.
• W2084790690 cites W2168041368 @default.
• W2084790690 cites W2185483792 @default.
• W2084790690 cites W2318855924 @default.
• W2084790690 cites W4237186561 @default.
• W2084790690 doi "https://doi.org/10.1186/1756-0500-6-10" @default.
• W2084790690 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3599066" @default.
• W2084790690 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23305096" @default.
• W2084790690 hasPublicationYear "2013" @default.
• W2084790690 type Work @default.
• W2084790690 sameAs 2084790690 @default.
• W2084790690 citedByCount "24" @default.
• W2084790690 countsByYear W20847906902013 @default.
• W2084790690 countsByYear W20847906902014 @default.
• W2084790690 countsByYear W20847906902015 @default.
• W2084790690 countsByYear W20847906902016 @default.
• W2084790690 countsByYear W20847906902017 @default.
• W2084790690 countsByYear W20847906902018 @default.
• W2084790690 countsByYear W20847906902019 @default.
• W2084790690 countsByYear W20847906902020 @default.
• W2084790690 countsByYear W20847906902021 @default.
• W2084790690 countsByYear W20847906902022 @default.
• W2084790690 countsByYear W20847906902023 @default.
• W2084790690 crossrefType "journal-article" @default.
• W2084790690 hasAuthorship W2084790690A5050143057 @default.
• W2084790690 hasAuthorship W2084790690A5076485897 @default.
• W2084790690 hasBestOaLocation W20847906901 @default.
• W2084790690 hasConcept C123012128 @default.
• W2084790690 hasConcept C12554922 @default.
• W2084790690 hasConcept C159985019 @default.
• W2084790690 hasConcept C17991360 @default.
• W2084790690 hasConcept C190283241 @default.
• W2084790690 hasConcept C192562407 @default.
• W2084790690 hasConcept C202751555 @default.
• W2084790690 hasConcept C203014093 @default.
• W2084790690 hasConcept C21141959 @default.
• W2084790690 hasConcept C55493867 @default.
• W2084790690 hasConcept C86803240 @default.
• W2084790690 hasConcept C95444343 @default.
• W2084790690 hasConceptScore W2084790690C123012128 @default.
• W2084790690 hasConceptScore W2084790690C12554922 @default.
• W2084790690 hasConceptScore W2084790690C159985019 @default.
• W2084790690 hasConceptScore W2084790690C17991360 @default.
• W2084790690 hasConceptScore W2084790690C190283241 @default.
• W2084790690 hasConceptScore W2084790690C192562407 @default.
• W2084790690 hasConceptScore W2084790690C202751555 @default.
• W2084790690 hasConceptScore W2084790690C203014093 @default.
• W2084790690 hasConceptScore W2084790690C21141959 @default.
• W2084790690 hasConceptScore W2084790690C55493867 @default.
• W2084790690 hasConceptScore W2084790690C86803240 @default.
• W2084790690 hasConceptScore W2084790690C95444343 @default.
• W2084790690 hasIssue "1" @default.
• W2084790690 hasLocation W20847906901 @default.
• W2084790690 hasLocation W20847906902 @default.
• W2084790690 hasLocation W20847906903 @default.
• W2084790690 hasLocation W20847906904 @default.
• W2084790690 hasOpenAccess W2084790690 @default.
• W2084790690 hasPrimaryLocation W20847906901 @default.
• W2084790690 hasRelatedWork W2000930640 @default.
• W2084790690 hasRelatedWork W2009251691 @default.
• W2084790690 hasRelatedWork W2034366718 @default.

• next