FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2084834123> ?p ?o ?g. }

• W2084834123 endingPage "1053" @default.
• W2084834123 startingPage "1044" @default.
• W2084834123 abstract "The gene encoding an esterase enzyme was cloned from a metagenomic library of cow rumen bacteria. The esterase gene (est5S) was 1,026 bp in length, encoding a protein of 366 amino acid residues with a calculated molecular mass of 40,168 Da. The molecular mass of the enzyme was estimated to be 40,000 Da. The Est5S protein contains the Gly-X-Ser-X-Gly motif found in most bacterial and eukaryotic serine hydrolases. However, the Asp or Glu necessary for the catalytic triad [Ser-Asp-(Glu)-His] was not present, indicating Est5S represents a novel member of the GHSQG family of esterolytic enzymes. BlastP in the NCBI database analysis of Est5S revealed homology to hypothetical proteins and it had no homology to previous known lipases and esterases. Est5S was optimally active at pH 7.0 and 40 degrees C. Among the p-nitrophenyl acylesters tested, high enzymatic activities were observed on the short-chain p-nitrophenyl acylesters, such as p-nitrophenyl acetate, etc. The conserved serine residue (Ser190) was shown to be important for Est5S activity. The primers that amplified the est5S gene did not show any relative band with 49 species of culturable rumen bacteria. This implies that a new group esterase gene, est5S, may have come from a noncultured cow rumen bacterium." @default.
• W2084834123 created "2016-06-24" @default.
• W2084834123 creator A5008214785 @default.
• W2084834123 creator A5022028069 @default.
• W2084834123 creator A5061650883 @default.
• W2084834123 creator A5064138676 @default.
• W2084834123 creator A5076147859 @default.
• W2084834123 date "2012-08-01" @default.
• W2084834123 modified "2023-09-29" @default.
• W2084834123 title "Cloning and Identification of a New Group Esterase (Est5S) from Noncultured Rumen Bacterium" @default.
• W2084834123 cites W1521148011 @default.
• W2084834123 cites W1561163642 @default.
• W2084834123 cites W1596665135 @default.
• W2084834123 cites W1966531892 @default.
• W2084834123 cites W1996618802 @default.
• W2084834123 cites W2001544957 @default.
• W2084834123 cites W2003071918 @default.
• W2084834123 cites W2005355029 @default.
• W2084834123 cites W2006211612 @default.
• W2084834123 cites W2009659777 @default.
• W2084834123 cites W2009782522 @default.
• W2084834123 cites W2010083706 @default.
• W2084834123 cites W2025294451 @default.
• W2084834123 cites W2040901564 @default.
• W2084834123 cites W2044646870 @default.
• W2084834123 cites W2055076888 @default.
• W2084834123 cites W2056213876 @default.
• W2084834123 cites W2063002060 @default.
• W2084834123 cites W2063479687 @default.
• W2084834123 cites W2069024116 @default.
• W2084834123 cites W2069616003 @default.
• W2084834123 cites W2071092023 @default.
• W2084834123 cites W2077174357 @default.
• W2084834123 cites W2085492900 @default.
• W2084834123 cites W2102077158 @default.
• W2084834123 cites W2103938843 @default.
• W2084834123 cites W2116142855 @default.
• W2084834123 cites W2126729834 @default.
• W2084834123 cites W2126905381 @default.
• W2084834123 cites W2137696890 @default.
• W2084834123 cites W2320290762 @default.
• W2084834123 cites W2414307244 @default.
• W2084834123 doi "https://doi.org/10.4014/jmb.1201.12070" @default.
• W2084834123 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22713979" @default.
• W2084834123 hasPublicationYear "2012" @default.
• W2084834123 type Work @default.
• W2084834123 sameAs 2084834123 @default.
• W2084834123 citedByCount "4" @default.
• W2084834123 countsByYear W20848341232015 @default.
• W2084834123 countsByYear W20848341232017 @default.
• W2084834123 countsByYear W20848341232021 @default.
• W2084834123 crossrefType "journal-article" @default.
• W2084834123 hasAuthorship W2084834123A5008214785 @default.
• W2084834123 hasAuthorship W2084834123A5022028069 @default.
• W2084834123 hasAuthorship W2084834123A5061650883 @default.
• W2084834123 hasAuthorship W2084834123A5064138676 @default.
• W2084834123 hasAuthorship W2084834123A5076147859 @default.
• W2084834123 hasConcept C100544194 @default.
• W2084834123 hasConcept C104317684 @default.
• W2084834123 hasConcept C121050878 @default.
• W2084834123 hasConcept C145770059 @default.
• W2084834123 hasConcept C165525559 @default.
• W2084834123 hasConcept C167625842 @default.
• W2084834123 hasConcept C181199279 @default.
• W2084834123 hasConcept C19924922 @default.
• W2084834123 hasConcept C199360897 @default.
• W2084834123 hasConcept C2776414213 @default.
• W2084834123 hasConcept C2777261171 @default.
• W2084834123 hasConcept C41008148 @default.
• W2084834123 hasConcept C515207424 @default.
• W2084834123 hasConcept C523546767 @default.
• W2084834123 hasConcept C54355233 @default.
• W2084834123 hasConcept C547475151 @default.
• W2084834123 hasConcept C55493867 @default.
• W2084834123 hasConcept C58121356 @default.
• W2084834123 hasConcept C70262065 @default.
• W2084834123 hasConcept C86803240 @default.
• W2084834123 hasConceptScore W2084834123C100544194 @default.
• W2084834123 hasConceptScore W2084834123C104317684 @default.
• W2084834123 hasConceptScore W2084834123C121050878 @default.
• W2084834123 hasConceptScore W2084834123C145770059 @default.
• W2084834123 hasConceptScore W2084834123C165525559 @default.
• W2084834123 hasConceptScore W2084834123C167625842 @default.
• W2084834123 hasConceptScore W2084834123C181199279 @default.
• W2084834123 hasConceptScore W2084834123C19924922 @default.
• W2084834123 hasConceptScore W2084834123C199360897 @default.
• W2084834123 hasConceptScore W2084834123C2776414213 @default.
• W2084834123 hasConceptScore W2084834123C2777261171 @default.
• W2084834123 hasConceptScore W2084834123C41008148 @default.
• W2084834123 hasConceptScore W2084834123C515207424 @default.
• W2084834123 hasConceptScore W2084834123C523546767 @default.
• W2084834123 hasConceptScore W2084834123C54355233 @default.
• W2084834123 hasConceptScore W2084834123C547475151 @default.
• W2084834123 hasConceptScore W2084834123C55493867 @default.
• W2084834123 hasConceptScore W2084834123C58121356 @default.
• W2084834123 hasConceptScore W2084834123C70262065 @default.
• W2084834123 hasConceptScore W2084834123C86803240 @default.
• W2084834123 hasIssue "8" @default.

• next