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- W2084842019 abstract "Abstract Passive hemagglutination using chromic chloride proved to be a rapid and useful method for a study of minute quantities of antigen extracted from larval Echinostoma lindoense (Sandground and Bonne), a trematode that develops in the snail intermediate host, Biomphalaria glabrata (Say). Parasite rediae were initially fragmented by three different procedures. Their soluble proteins were separated into two bands by electrophoresis on cellulose acetate, and into three fractions by molecular sieve chromatography. Rabbit antiserum was prepared from six weekly intramuscular injections of soluble redial protein in complete Freund's adjuvant, followed after 1 month by a single inoculation of alum-precipitated antigen. Antiserum was absorbed free of anti-snail antibodies and the immune complexes were removed by ion-exchange chromatography over DEAE-cellulose, producing an immunochemically pure IgG. Study of the rabbit anti-trematode antibody by precipitation, complement fixation, hemagglutination (HA), and inhibition of HA revealed a specific and high titered anti-larval antibody. These methods offer an approach to the problem of measuring the snail host's protective response against trematode reinfection; they also can be used to study the antigenic maturation of successive larval stages in the intermediate host." @default.
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- W2084842019 date "1971-06-01" @default.
- W2084842019 modified "2023-10-17" @default.
- W2084842019 title "Echinostoma lindoense: Larval antigens from the snail intermediate host, Biomphalaria glabrata" @default.
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- W2084842019 doi "https://doi.org/10.1016/0014-4894(71)90057-9" @default.
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