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• W2084917929 abstract "Acylpeptide hydrolase was purified to homogeneity from porcine intestinal mucosa using a seven-step procedure including ammonium sulfate precipitation, gel filtration as well as anion exchange and affinity chromatography. The specific activity of the enzyme reached 105 000 nmol/mg protein per min and the purification was as high as 5500-fold. This tetrameric enzyme is composed of four apparently identical subunits, the molecular mass of which was estimated to be 75 kDa, based on the results of amino acid analysis and gel electrophoresis performed under denaturing conditions. It is likely that the NH2-terminal residue may be acetylated, while serine was found to be the COOH-terminal residue. The hydrolytic activity of the enzyme toward N-acetyl-l-alanine p-nitroanilide at the optimum pH value was increased twofold in the presence of the chloride anion. The Km value calculated from the kinetics of the hydrolysis of acetylalanyl peptides was found to be 0.7±0.1 mM, whereas the Vmax value decreased from 200 to 50 nmol/min per μg of enzyme, depending on the peptidic chain lengths. The Vmax value of the synthetic substrate (250 nmol/min per μg of enzyme) was 25–500% higher than those of the acetylalanyl peptides, depending on the peptide chain length, although the enzyme affinity was slightly lower (1.8 mM as compared with 0.7 mM). In line with data on other animal species and on various tissues, the enzyme seemed likely to be a serine protease, since it was readily inhibited by diisopropyl fluorophosphate and diethyl pyrocarbonate. A 2377-nucleotide long cDNA coding for the enzyme was isolated from pig small intestine. The deduced amino acid sequence consisted of 731 residues and showed a single different amino acid with that of the porcine liver APH, except the N-terminal amino acid which is still probably lacking." @default.
• W2084917929 created "2016-06-24" @default.
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• W2084917929 date "1999-07-01" @default.
• W2084917929 modified "2023-10-18" @default.
• W2084917929 title "Cloning, sequencing and further characterization of acylpeptide hydrolase from porcine intestinal mucosa" @default.
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• W2084917929 doi "https://doi.org/10.1016/s0167-4838(99)00087-4" @default.
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