Matches in SemOpenAlex for { <https://semopenalex.org/work/W2084928618> ?p ?o ?g. }
- W2084928618 endingPage "236" @default.
- W2084928618 startingPage "227" @default.
- W2084928618 abstract "Cultured neurons from the paravertebral sympathetic chain of rats possess excitatory P2X as well as excitatory uracil nucleotide-sensitive P2Y receptors. Preliminary observations had indicated that the analogous neurons of mice lacked P2X receptors. This difference was now investigated. Thoracolumbar sympathetic neurons from one- to three-day-old mice were cultured for seven days. When the neurons were preincubated with [3H]noradrenaline and then superfused, ATP failed to cause any change in tritium outflow. UTP (3-300 microM) and UDP (30-100 microM), in contrast, caused marked increases, and so did nicotine (3-100 microM). The effect of UTP was not changed by suramin but abolished by tetrodotoxin and in the absence of calcium. The effect of nicotine was antagonized by hexamethonium and also abolished by tetrodotoxin and in the absence of calcium. Pre-exposure to UDP prevented the effect of UTP. In neurons studied by means of whole-cell patch-clamp techniques under current clamp, ATP lacked any effect. UTP (100 microM), UDP (100 microM) and nicotine (10 microM) caused depolarization accompanied by action potentials. Pre-exposure to UDP prevented the effect of UTP. In neurons studied under voltage clamp, ATP, UTP and UDP failed to cause any detectable current. Nicotine (10 microM), in contrast, elicited inward currents. Neither UTP nor UDP reduced the M-type potassium outward current. These results demonstrate a pronounced difference between cultured sympathetic neurons from the mouse and the rat paravertebral chain. Neurons from both species possess the nicotinic acetylcholine receptor. Neurons from both species also possess uracil nucleotide-sensitive P2Y receptors which, when activated, mediate depolarization, action potential firing and noradrenaline release; these effects are not due to inhibition of M-type potassium channels. Only the rat but not the mouse neurons, however, possess P2X receptors which, when activated, mediate cation entry, depolarization, action potential generation and transmitter release. The absence of functional P2X receptors makes the mouse neurons suitable for further study of the uracil nucleotide-sensitive P2Y receptors." @default.
- W2084928618 created "2016-06-24" @default.
- W2084928618 creator A5007905634 @default.
- W2084928618 creator A5008390848 @default.
- W2084928618 creator A5049724951 @default.
- W2084928618 creator A5056648436 @default.
- W2084928618 creator A5056835151 @default.
- W2084928618 creator A5060222373 @default.
- W2084928618 date "2001-02-01" @default.
- W2084928618 modified "2023-10-16" @default.
- W2084928618 title "Stimulation of mouse cultured sympathetic neurons by uracil but not adenine nucleotides" @default.
- W2084928618 cites W1498663323 @default.
- W2084928618 cites W1505417280 @default.
- W2084928618 cites W1507602988 @default.
- W2084928618 cites W1512303177 @default.
- W2084928618 cites W1564218032 @default.
- W2084928618 cites W1972165417 @default.
- W2084928618 cites W1993350951 @default.
- W2084928618 cites W2000033837 @default.
- W2084928618 cites W2003026743 @default.
- W2084928618 cites W2004427424 @default.
- W2084928618 cites W2028236212 @default.
- W2084928618 cites W2028832749 @default.
- W2084928618 cites W2029100084 @default.
- W2084928618 cites W2034163631 @default.
- W2084928618 cites W2039102040 @default.
- W2084928618 cites W2051649981 @default.
- W2084928618 cites W2065939463 @default.
- W2084928618 cites W2071248579 @default.
- W2084928618 cites W2078075016 @default.
- W2084928618 cites W2081672847 @default.
- W2084928618 cites W2082978448 @default.
- W2084928618 cites W2083438878 @default.
- W2084928618 cites W2090395826 @default.
- W2084928618 cites W2117946812 @default.
- W2084928618 cites W2131509875 @default.
- W2084928618 cites W2132298210 @default.
- W2084928618 cites W2133951476 @default.
- W2084928618 doi "https://doi.org/10.1016/s0306-4522(00)00547-9" @default.
- W2084928618 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11311803" @default.
- W2084928618 hasPublicationYear "2001" @default.
- W2084928618 type Work @default.
- W2084928618 sameAs 2084928618 @default.
- W2084928618 citedByCount "12" @default.
- W2084928618 countsByYear W20849286182012 @default.
- W2084928618 countsByYear W20849286182015 @default.
- W2084928618 countsByYear W20849286182023 @default.
- W2084928618 crossrefType "journal-article" @default.
- W2084928618 hasAuthorship W2084928618A5007905634 @default.
- W2084928618 hasAuthorship W2084928618A5008390848 @default.
- W2084928618 hasAuthorship W2084928618A5049724951 @default.
- W2084928618 hasAuthorship W2084928618A5056648436 @default.
- W2084928618 hasAuthorship W2084928618A5056835151 @default.
- W2084928618 hasAuthorship W2084928618A5060222373 @default.
- W2084928618 hasConcept C112592302 @default.
- W2084928618 hasConcept C126322002 @default.
- W2084928618 hasConcept C134018914 @default.
- W2084928618 hasConcept C147944092 @default.
- W2084928618 hasConcept C170493617 @default.
- W2084928618 hasConcept C17077164 @default.
- W2084928618 hasConcept C185592680 @default.
- W2084928618 hasConcept C24998067 @default.
- W2084928618 hasConcept C2775893369 @default.
- W2084928618 hasConcept C2775910092 @default.
- W2084928618 hasConcept C2776182878 @default.
- W2084928618 hasConcept C2776741303 @default.
- W2084928618 hasConcept C2778993773 @default.
- W2084928618 hasConcept C4141045 @default.
- W2084928618 hasConcept C71924100 @default.
- W2084928618 hasConcept C86803240 @default.
- W2084928618 hasConceptScore W2084928618C112592302 @default.
- W2084928618 hasConceptScore W2084928618C126322002 @default.
- W2084928618 hasConceptScore W2084928618C134018914 @default.
- W2084928618 hasConceptScore W2084928618C147944092 @default.
- W2084928618 hasConceptScore W2084928618C170493617 @default.
- W2084928618 hasConceptScore W2084928618C17077164 @default.
- W2084928618 hasConceptScore W2084928618C185592680 @default.
- W2084928618 hasConceptScore W2084928618C24998067 @default.
- W2084928618 hasConceptScore W2084928618C2775893369 @default.
- W2084928618 hasConceptScore W2084928618C2775910092 @default.
- W2084928618 hasConceptScore W2084928618C2776182878 @default.
- W2084928618 hasConceptScore W2084928618C2776741303 @default.
- W2084928618 hasConceptScore W2084928618C2778993773 @default.
- W2084928618 hasConceptScore W2084928618C4141045 @default.
- W2084928618 hasConceptScore W2084928618C71924100 @default.
- W2084928618 hasConceptScore W2084928618C86803240 @default.
- W2084928618 hasIssue "1" @default.
- W2084928618 hasLocation W20849286181 @default.
- W2084928618 hasLocation W20849286182 @default.
- W2084928618 hasOpenAccess W2084928618 @default.
- W2084928618 hasPrimaryLocation W20849286181 @default.
- W2084928618 hasRelatedWork W1976128502 @default.
- W2084928618 hasRelatedWork W1992805685 @default.
- W2084928618 hasRelatedWork W1995226963 @default.
- W2084928618 hasRelatedWork W2031043443 @default.
- W2084928618 hasRelatedWork W2043765423 @default.
- W2084928618 hasRelatedWork W2048194509 @default.
- W2084928618 hasRelatedWork W2054992011 @default.