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• W2084954029 abstract "The photoreduction of protochlorophyllide to chlorophyllide in holochrome preparations or homogenates from etiolated bean seedlings (Phaseolus vulgaris L.) is followed by a series of dark steps involving changes in the absorption and circular dichroism spectra and in the polarization of the chlorophyllide fluorescence at 7 °C. Related changes can be seen in the pigment-protein, protochlorophyllide holochrome, isolated from bean etioplasts. The circular dichroism and fluorescence polarization spectra, which are particularly sensitive to close pigment association, suggest that the unilluminated holochrome contains protochlorophyllide a in an aggregated form, probably as dimers. Illumination produces a chlorophyllide a holochrome which initially exhibits evidence of chlorophyll aggregation, but which undergoes dissociation to perhaps a monomeric form within a few minutes at 0 °C. If etiolated leaves are illuminated for periods longer than 1 h, they then appear to accumulate chlorophyll b and chlorophyll a in aggregated forms that are distinct from one another. The relevance of these findings to the development of photosynthetically active plastid membranes is discussed." @default.
• W2084954029 created "2016-06-24" @default.
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• W2084954029 date "1972-05-01" @default.
• W2084954029 modified "2023-09-26" @default.
• W2084954029 title "Circular dichroism and fluorescence changes accompanying the protochlorophyllide to chlorophyllide transformation in greening leaves and holochrome preparations" @default.
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• W2084954029 doi "https://doi.org/10.1016/0005-2728(72)90120-x" @default.
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