FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2085019148> ?p ?o ?g. }

• W2085019148 endingPage "A105" @default.
• W2085019148 startingPage "A105.2" @default.
• W2085019148 abstract "T-cells are recruited from the blood into extra-vascular tissues during acute inflammation. However, in chronic inflammatory diseases, including atherosclerosis, an inappropriate accumulation of T-cells in the diseased tissue contributes to pathogenesis. Very little is known about the mechanisms by which T-cell trafficking is regulated during inflammation, and it is thus difficult to target this aspect of pathology for the development of new anti-atherogenic therapies. Here we describe a unique immune regulatory peptide that imposes a tonic inhibition of T-cell trafficking during inflammation. PEPtide Inhibitor of Trans-Endothelial Migration (PEPITEM) introduces a new paradigm into the pathways that regulate the inflammatory response. We propose that loss of this regulatory pathway makes the immune system ‘leaky’, allowing inappropriate access of T-cells to vulnerable tissues in chronic diseases. Lymphocyte trafficking was assessed <i>in vitro</i> using videomicroscopy on TNF-α/IFN-γ activated endothelial cells (EC) and lymphocytes isolated from healthy donors or patients with chronic inflammatory disease. <i>In vivo</i>, lymphocyte recruitment was assessed in a model of zymosan-driven peritoneal inflammation. PEPITEM was identified using mass spectrometry. Our studies began with an interest in adiponectin, an anti-inflammatory adipose tissue-derived cytokine. Using an <i>in vitro</i> migration assay, we observed that the migration of human lymphocytes was dose-dependently blocked by adiponectin. Adiponectin achieves its effects on T-cell migration by the induction of a novel mediator released from B-cells. Thus, the effect of adiponectin was lost when B cells are absent, but could be regained by the addition of supernatants from adiponectin stimulated B-cells. Interestingly, the B-cell derived product did not act directly on T-cells; rather, it stimulated EC to release the lipid mediator sphingosine-1-phosphate, which in turn inhibited the migration of T-cells. We used mass spectrometry to isolate a B-cell derived peptide, corresponding uniquely in the human genome to a proteolytic excision product of the 14.3.3ζδ protein. Synthetic PEPITEM could also effectively inhibit T-cell migration. In zymosan-induced peritonitis in the mouse, T-cell recruitment was significantly increased in a strain lacking B cells when compared to wild-type animals. This excess of T-cell recruitment was ameliorated by treatment with PEPITEM. Lymphocytes isolated from patients with chronic inflammatory disease (type-1-diabetes) were released from the inhibitory effects of adiponectin, but this regulatory pathway could be re-established by the addition of exogenous PEPITEM. We believe that PEPITEM and its associated pathway may have therapeutic efficacy in a number of disease scenarios including atherosclerosis." @default.
• W2085019148 created "2016-06-24" @default.
• W2085019148 creator A5010548890 @default.
• W2085019148 creator A5012560667 @default.
• W2085019148 creator A5026318124 @default.
• W2085019148 creator A5028825989 @default.
• W2085019148 creator A5037259385 @default.
• W2085019148 creator A5043119059 @default.
• W2085019148 creator A5071833047 @default.
• W2085019148 creator A5078588605 @default.
• W2085019148 creator A5084277158 @default.
• W2085019148 creator A5087851003 @default.
• W2085019148 date "2013-05-01" @default.
• W2085019148 modified "2023-10-16" @default.
• W2085019148 title "187 THE PEPTIDE INHIBITOR OF TRANS-ENDOTHELIAL MIGRATION, PEPITEM, A NOVEL IMMUNE REGUALTORY AGENT, CONTROLS T-CELL TRAFFICKING DURING INFLAMMATION, A TONIC INHIBITORY PATHWAY THAT IS LOST IN CHRONIC DISEASE" @default.
• W2085019148 doi "https://doi.org/10.1136/heartjnl-2013-304019.187" @default.
• W2085019148 hasPublicationYear "2013" @default.
• W2085019148 type Work @default.
• W2085019148 sameAs 2085019148 @default.
• W2085019148 citedByCount "0" @default.
• W2085019148 crossrefType "journal-article" @default.
• W2085019148 hasAuthorship W2085019148A5010548890 @default.
• W2085019148 hasAuthorship W2085019148A5012560667 @default.
• W2085019148 hasAuthorship W2085019148A5026318124 @default.
• W2085019148 hasAuthorship W2085019148A5028825989 @default.
• W2085019148 hasAuthorship W2085019148A5037259385 @default.
• W2085019148 hasAuthorship W2085019148A5043119059 @default.
• W2085019148 hasAuthorship W2085019148A5071833047 @default.
• W2085019148 hasAuthorship W2085019148A5078588605 @default.
• W2085019148 hasAuthorship W2085019148A5084277158 @default.
• W2085019148 hasAuthorship W2085019148A5087851003 @default.
• W2085019148 hasBestOaLocation W20850191481 @default.
• W2085019148 hasConcept C123012128 @default.
• W2085019148 hasConcept C134018914 @default.
• W2085019148 hasConcept C202751555 @default.
• W2085019148 hasConcept C203014093 @default.
• W2085019148 hasConcept C2776090121 @default.
• W2085019148 hasConcept C2776782570 @default.
• W2085019148 hasConcept C2776914184 @default.
• W2085019148 hasConcept C2777391703 @default.
• W2085019148 hasConcept C2778690821 @default.
• W2085019148 hasConcept C2780942790 @default.
• W2085019148 hasConcept C55493867 @default.
• W2085019148 hasConcept C555293320 @default.
• W2085019148 hasConcept C71924100 @default.
• W2085019148 hasConcept C86803240 @default.
• W2085019148 hasConcept C8891405 @default.
• W2085019148 hasConcept C95444343 @default.
• W2085019148 hasConceptScore W2085019148C123012128 @default.
• W2085019148 hasConceptScore W2085019148C134018914 @default.
• W2085019148 hasConceptScore W2085019148C202751555 @default.
• W2085019148 hasConceptScore W2085019148C203014093 @default.
• W2085019148 hasConceptScore W2085019148C2776090121 @default.
• W2085019148 hasConceptScore W2085019148C2776782570 @default.
• W2085019148 hasConceptScore W2085019148C2776914184 @default.
• W2085019148 hasConceptScore W2085019148C2777391703 @default.
• W2085019148 hasConceptScore W2085019148C2778690821 @default.
• W2085019148 hasConceptScore W2085019148C2780942790 @default.
• W2085019148 hasConceptScore W2085019148C55493867 @default.
• W2085019148 hasConceptScore W2085019148C555293320 @default.
• W2085019148 hasConceptScore W2085019148C71924100 @default.
• W2085019148 hasConceptScore W2085019148C86803240 @default.
• W2085019148 hasConceptScore W2085019148C8891405 @default.
• W2085019148 hasConceptScore W2085019148C95444343 @default.
• W2085019148 hasIssue "suppl 2" @default.
• W2085019148 hasLocation W20850191481 @default.
• W2085019148 hasOpenAccess W2085019148 @default.
• W2085019148 hasPrimaryLocation W20850191481 @default.
• W2085019148 hasRelatedWork W1993985418 @default.
• W2085019148 hasRelatedWork W2047209980 @default.
• W2085019148 hasRelatedWork W2186316345 @default.
• W2085019148 hasRelatedWork W2313447129 @default.
• W2085019148 hasRelatedWork W2473304459 @default.
• W2085019148 hasRelatedWork W3030458261 @default.
• W2085019148 hasRelatedWork W3136031961 @default.
• W2085019148 hasRelatedWork W4248746885 @default.
• W2085019148 hasRelatedWork W2185732136 @default.
• W2085019148 hasRelatedWork W2396811992 @default.
• W2085019148 hasVolume "99" @default.
• W2085019148 isParatext "false" @default.
• W2085019148 isRetracted "false" @default.
• W2085019148 magId "2085019148" @default.
• W2085019148 workType "article" @default.