Matches in SemOpenAlex for { <https://semopenalex.org/work/W2085025163> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W2085025163 abstract "Leukotriene B4 (LTB4) from vascular endothelium may play a key role in the genesis of atherosclerotic lesions. However, the ability of this tissue to synthesize LTB4 is controversial. To resolve this issue arachidonic acid metabolism was characterized in cultures of confluent monolayers of a rabbit aortic endothelial cell line by use of both high-pressure liquid chromatography and radioimmunoassay. Cells were grown to confluence in Dulbecco's modified Eagle's medium/Ham's F12 with 5% fetal bovine serum. Lipoxygenase activity was studied by placing the cells in Hank's balanced salt solution with 2 mumol/L indomethacin. After 30 minutes preincubation with indomethacin cells were exposed to either arachidonic acid (10 mumol/L) or arachidonic acid labeled with radioactive carbon (14C) (1 microCi; SA 58 mCi/mmol) and then stimulated with 9.5 mumol/L calcium ionophore A23187 for 55 minutes. Studies of the cyclooxygenase activity were performed without preincubating with indomethacin. Samples were prepared for high-pressure liquid chromatography by evaporation to dryness under a vacuum and resuspending in 2 ml of 1:1 methanol/water. Tritium-labeled standards were added before loading the 14C-labeled samples on the column. Radiolabeled arachidonic acid metabolites were separated by high-pressure liquid chromatography and detected by means of a dual channel flow-through radiodetector that monitors both 14C and 3H. Based on coelution with authentic standards three lipoxygenase metabolites of arachidonic acid have been identified: LTB4, 12- and 5-hydroxyeicosatetraneoic acid. Leukotriene B4 was further characterized by ultraviolet spectral analysis and inhibition studies with use of nordihydroguaiaretic acid. Quantitation was facilitated by commercially available radioimmunoassay kits. An average of 600 pg LTB4/10(6) cells was measured from separate experiments.(ABSTRACT TRUNCATED AT 250 WORDS)" @default.
- W2085025163 created "2016-06-24" @default.
- W2085025163 creator A5008904034 @default.
- W2085025163 creator A5031619640 @default.
- W2085025163 creator A5039322083 @default.
- W2085025163 creator A5082495393 @default.
- W2085025163 creator A5089583945 @default.
- W2085025163 date "1990-09-01" @default.
- W2085025163 modified "2023-09-24" @default.
- W2085025163 title "Endothelial cells can synthesize leukotriene B4" @default.
- W2085025163 cites W1985153176 @default.
- W2085025163 cites W1987146416 @default.
- W2085025163 cites W1998562858 @default.
- W2085025163 cites W2005115885 @default.
- W2085025163 cites W2023213942 @default.
- W2085025163 cites W2024065582 @default.
- W2085025163 cites W2047277639 @default.
- W2085025163 cites W2049796670 @default.
- W2085025163 cites W2057758369 @default.
- W2085025163 cites W2058302661 @default.
- W2085025163 cites W2085885618 @default.
- W2085025163 cites W2135231814 @default.
- W2085025163 cites W2198257851 @default.
- W2085025163 cites W2419364493 @default.
- W2085025163 doi "https://doi.org/10.1016/0741-5214(90)90151-y" @default.
- W2085025163 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2169005" @default.
- W2085025163 hasPublicationYear "1990" @default.
- W2085025163 type Work @default.
- W2085025163 sameAs 2085025163 @default.
- W2085025163 citedByCount "6" @default.
- W2085025163 countsByYear W20850251632013 @default.
- W2085025163 crossrefType "journal-article" @default.
- W2085025163 hasAuthorship W2085025163A5008904034 @default.
- W2085025163 hasAuthorship W2085025163A5031619640 @default.
- W2085025163 hasAuthorship W2085025163A5039322083 @default.
- W2085025163 hasAuthorship W2085025163A5082495393 @default.
- W2085025163 hasAuthorship W2085025163A5089583945 @default.
- W2085025163 hasBestOaLocation W20850251631 @default.
- W2085025163 hasConcept C126322002 @default.
- W2085025163 hasConcept C176685330 @default.
- W2085025163 hasConcept C178790620 @default.
- W2085025163 hasConcept C181199279 @default.
- W2085025163 hasConcept C185592680 @default.
- W2085025163 hasConcept C2776042228 @default.
- W2085025163 hasConcept C2776452501 @default.
- W2085025163 hasConcept C2776685179 @default.
- W2085025163 hasConcept C2776914184 @default.
- W2085025163 hasConcept C2778078955 @default.
- W2085025163 hasConcept C2778996431 @default.
- W2085025163 hasConcept C2779630222 @default.
- W2085025163 hasConcept C2780857362 @default.
- W2085025163 hasConcept C43617362 @default.
- W2085025163 hasConcept C55493867 @default.
- W2085025163 hasConcept C71924100 @default.
- W2085025163 hasConceptScore W2085025163C126322002 @default.
- W2085025163 hasConceptScore W2085025163C176685330 @default.
- W2085025163 hasConceptScore W2085025163C178790620 @default.
- W2085025163 hasConceptScore W2085025163C181199279 @default.
- W2085025163 hasConceptScore W2085025163C185592680 @default.
- W2085025163 hasConceptScore W2085025163C2776042228 @default.
- W2085025163 hasConceptScore W2085025163C2776452501 @default.
- W2085025163 hasConceptScore W2085025163C2776685179 @default.
- W2085025163 hasConceptScore W2085025163C2776914184 @default.
- W2085025163 hasConceptScore W2085025163C2778078955 @default.
- W2085025163 hasConceptScore W2085025163C2778996431 @default.
- W2085025163 hasConceptScore W2085025163C2779630222 @default.
- W2085025163 hasConceptScore W2085025163C2780857362 @default.
- W2085025163 hasConceptScore W2085025163C43617362 @default.
- W2085025163 hasConceptScore W2085025163C55493867 @default.
- W2085025163 hasConceptScore W2085025163C71924100 @default.
- W2085025163 hasLocation W20850251631 @default.
- W2085025163 hasLocation W20850251632 @default.
- W2085025163 hasOpenAccess W2085025163 @default.
- W2085025163 hasPrimaryLocation W20850251631 @default.
- W2085025163 hasRelatedWork W123069601 @default.
- W2085025163 hasRelatedWork W1879285787 @default.
- W2085025163 hasRelatedWork W1973446102 @default.
- W2085025163 hasRelatedWork W1979457780 @default.
- W2085025163 hasRelatedWork W2011670818 @default.
- W2085025163 hasRelatedWork W2052320154 @default.
- W2085025163 hasRelatedWork W2069092878 @default.
- W2085025163 hasRelatedWork W2079329017 @default.
- W2085025163 hasRelatedWork W2085025163 @default.
- W2085025163 hasRelatedWork W2419193380 @default.
- W2085025163 isParatext "false" @default.
- W2085025163 isRetracted "false" @default.
- W2085025163 magId "2085025163" @default.
- W2085025163 workType "article" @default.