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• W2085038512 abstract "Localisation microscopy methods allow to realize a light optical resolution far beyond the Abbe-Rayleigh limit of about 200 nm laterally and 600 nm axially. So far, this progress was achieved using labelling with appropriate fluorochromes and fluorescent proteins. Here, we describe for the first time that optical resolution of cellular structures in the λ/10 range (∼50 nm) can be achieved even in label-free cells. This was obtained using Spectral Precision Distance/Position Determination Microscopy (SPDM), a method based on the general principles of localisation microscopy. Besides a substantial resolution improvement of autofluorescent structures, SPDM revealed cellular objects which are not detectable under conventional fluorescence imaging conditions." @default.
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• W2085038512 date "2011-06-01" @default.
• W2085038512 modified "2023-09-26" @default.
• W2085038512 title "Imaging label-free intracellular structures by localisation microscopy" @default.
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• W2085038512 doi "https://doi.org/10.1016/j.micron.2010.03.006" @default.
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