FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2085105418> ?p ?o ?g. }

• W2085105418 endingPage "448" @default.
• W2085105418 startingPage "439" @default.
• W2085105418 abstract "In the present study, we examined the role of PLCδ1 (phospholipase C δ1) in the regulation of cellular proliferation. We demonstrate that RNAi (RNA interference)-mediated knockdown of endogenous PLCδ1, but not PLCβ3 or PLCϵ, induces a proliferation defect in Rat-1 and NIH 3T3 fibroblasts. The decreased proliferation was not due to an induction of apoptosis or senescence, but was associated with an approx. 60% inhibition of [3H]thymidine incorporation. Analysis of the cell cycle with BrdU (bromodeoxyuridine)/propidium iodide-labelled FACS (fluorescence-activated cell sorting) demonstrated an accumulation of cells in G0/G1-phase and a corresponding decrease in cells in S-phase. Further examination of the cell cycle after synchronization by serum-starvation demonstrated normal movement through G1-phase but delayed entry into S-phase. Consistent with these findings, G1 cyclin (D2 and D3) and CDK4 (cyclin-dependent kinase 4) levels and associated kinase activity were not affected. However, cyclin E-associated CDK2 activity, responsible for G1-to-S-phase progression, was inhibited. This decreased activity was accompanied by unchanged CDK2 protein levels and paradoxically elevated cyclin E and cyclin E-associated CDK2 levels, suggesting inhibition of the cyclin E–CDK2 complex. This inhibition was not due to altered stimulatory or inhibitory phosphorylation of CDK2. However, p27, a Cip/Kip family CKI (CDK inhibitor)-binding partner, was elevated and showed increased association with CDK2 in PLCδ1-knockdown cells. The result of the present study demonstrate a novel and critical role for PLCδ1 in cell-cycle progression from G1-to-S-phase through regulation of cyclin E–CDK2 activity and p27 levels." @default.
• W2085105418 created "2016-06-24" @default.
• W2085105418 creator A5031006563 @default.
• W2085105418 creator A5060391320 @default.
• W2085105418 creator A5074460588 @default.
• W2085105418 creator A5091014052 @default.
• W2085105418 date "2008-10-15" @default.
• W2085105418 modified "2023-09-27" @default.
• W2085105418 title "Phospholipase Cδ1 regulates cell proliferation and cell-cycle progression from G1- to S-phase by control of cyclin E–CDK2 activity" @default.
• W2085105418 cites W1554170959 @default.
• W2085105418 cites W1967336624 @default.
• W2085105418 cites W1970779989 @default.
• W2085105418 cites W1974821132 @default.
• W2085105418 cites W1984012038 @default.
• W2085105418 cites W1995590506 @default.
• W2085105418 cites W2003190738 @default.
• W2085105418 cites W2010972042 @default.
• W2085105418 cites W2016626397 @default.
• W2085105418 cites W2028144722 @default.
• W2085105418 cites W2032456072 @default.
• W2085105418 cites W2034333385 @default.
• W2085105418 cites W2038783205 @default.
• W2085105418 cites W2044312503 @default.
• W2085105418 cites W2046919270 @default.
• W2085105418 cites W2049097459 @default.
• W2085105418 cites W2057227495 @default.
• W2085105418 cites W2068344246 @default.
• W2085105418 cites W2069699841 @default.
• W2085105418 cites W2083705213 @default.
• W2085105418 cites W2085693261 @default.
• W2085105418 cites W2088085878 @default.
• W2085105418 cites W2096549360 @default.
• W2085105418 cites W2099936575 @default.
• W2085105418 cites W2100117790 @default.
• W2085105418 cites W2110346630 @default.
• W2085105418 cites W2121619780 @default.
• W2085105418 cites W2131303996 @default.
• W2085105418 cites W2140484163 @default.
• W2085105418 cites W2152398347 @default.
• W2085105418 cites W2169927110 @default.
• W2085105418 cites W4239524546 @default.
• W2085105418 doi "https://doi.org/10.1042/bj20080233" @default.
• W2085105418 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18588506" @default.
• W2085105418 hasPublicationYear "2008" @default.
• W2085105418 type Work @default.
• W2085105418 sameAs 2085105418 @default.
• W2085105418 citedByCount "19" @default.
• W2085105418 countsByYear W20851054182012 @default.
• W2085105418 countsByYear W20851054182013 @default.
• W2085105418 countsByYear W20851054182014 @default.
• W2085105418 countsByYear W20851054182016 @default.
• W2085105418 countsByYear W20851054182018 @default.
• W2085105418 countsByYear W20851054182019 @default.
• W2085105418 countsByYear W20851054182020 @default.
• W2085105418 countsByYear W20851054182021 @default.
• W2085105418 countsByYear W20851054182023 @default.
• W2085105418 crossrefType "journal-article" @default.
• W2085105418 hasAuthorship W2085105418A5031006563 @default.
• W2085105418 hasAuthorship W2085105418A5060391320 @default.
• W2085105418 hasAuthorship W2085105418A5074460588 @default.
• W2085105418 hasAuthorship W2085105418A5091014052 @default.
• W2085105418 hasConcept C105696609 @default.
• W2085105418 hasConcept C11882975 @default.
• W2085105418 hasConcept C120089663 @default.
• W2085105418 hasConcept C124320809 @default.
• W2085105418 hasConcept C1491633281 @default.
• W2085105418 hasConcept C152857842 @default.
• W2085105418 hasConcept C153911025 @default.
• W2085105418 hasConcept C16438837 @default.
• W2085105418 hasConcept C184235292 @default.
• W2085105418 hasConcept C25311885 @default.
• W2085105418 hasConcept C28179978 @default.
• W2085105418 hasConcept C29537977 @default.
• W2085105418 hasConcept C55493867 @default.
• W2085105418 hasConcept C62112901 @default.
• W2085105418 hasConcept C82495950 @default.
• W2085105418 hasConcept C86803240 @default.
• W2085105418 hasConcept C94561458 @default.
• W2085105418 hasConcept C95444343 @default.
• W2085105418 hasConcept C97029542 @default.
• W2085105418 hasConcept C9743823 @default.
• W2085105418 hasConceptScore W2085105418C105696609 @default.
• W2085105418 hasConceptScore W2085105418C11882975 @default.
• W2085105418 hasConceptScore W2085105418C120089663 @default.
• W2085105418 hasConceptScore W2085105418C124320809 @default.
• W2085105418 hasConceptScore W2085105418C1491633281 @default.
• W2085105418 hasConceptScore W2085105418C152857842 @default.
• W2085105418 hasConceptScore W2085105418C153911025 @default.
• W2085105418 hasConceptScore W2085105418C16438837 @default.
• W2085105418 hasConceptScore W2085105418C184235292 @default.
• W2085105418 hasConceptScore W2085105418C25311885 @default.
• W2085105418 hasConceptScore W2085105418C28179978 @default.
• W2085105418 hasConceptScore W2085105418C29537977 @default.
• W2085105418 hasConceptScore W2085105418C55493867 @default.
• W2085105418 hasConceptScore W2085105418C62112901 @default.
• W2085105418 hasConceptScore W2085105418C82495950 @default.
• W2085105418 hasConceptScore W2085105418C86803240 @default.
• W2085105418 hasConceptScore W2085105418C94561458 @default.

• next