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- W2085143896 abstract "The bacteriophage PM2 requires extracellular Ca2+ at concentrations greater than 3 · 10−4 M for the production of viable virus, whereas the host cell Pseudomonas BAL-31 grows normally in medium containing 3 · 10−5 M Ca2+ (low calcium). Virus attachment occurs normally in low calcium, the infected cultures partially lyse, but no infectious virus particles are released. Sucrose gradient analysis shows that lysates made in low calcium contain no PM2-like particles. The addition of calcium very late in the infectious cycle completely restores virus production to cultures infected in low calcium, whereas removal of calcium after infection prevents virus production. Our experiments indicate that Ca2+ is essential for some process late in the lytic cycle, such as the final assembly of stable, infectious PM2 particles." @default.
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- W2085143896 date "1974-03-01" @default.
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- W2085143896 title "Calcium requirement for assembly of the lipid-containing bacteriophage PM2" @default.
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- W2085143896 doi "https://doi.org/10.1016/0005-2736(74)90158-8" @default.
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